Purification and Application of Chlamydia trachomatis Effector CTL0398

Authors

Zachary E. Wagner, University of Central FloridaFollow
Kenneth Garcia, University of Central FloridaFollow

Streaming Media

Files

Course Code

MCB

Course Number

4920C

Faculty/Instructor

George Aranjuez

Faculty/Instructor Email

George.Aranjuez@ucf.edu

About the Author

My name is Zachary Wagner, I am a 4th year biomedical sciences student. Kenneth Garcia is a 3rd year biomedical sciences student. We both took GEAR (MCB 4920C), in fall 2024.

Abstract, Summary, or Creative Statement

Chlamydia trachomatis is a bacterial pathogen which utilizes secretion of effector proteins which target host cellular processes, while evading the host immune system. Using the pUAST vector and the GAL4/UAS expression system, it is possible to enhance the expression of any select Chlamydia protein effector. We took steps to purify one of these effectors, CTL0398, through PCR amplification, Gel Electrophoresis, and Colony PCR. These procedures were done with the hopes of future studies uncovering the true function of the select effector, and if there is any manifested phenotypic change in drosophila.

Keywords

Drosophila; Chlamydia; trachomatis

Recommended Citation

Wagner, Zachary E. and Garcia, Kenneth, "Purification and Application of Chlamydia trachomatis Effector CTL0398" (2025). High Impact Practices Student Showcase Spring 2025. 15.
https://stars.library.ucf.edu/hip-2025spring/15