High Impact Practices Student Showcase Spring 2025
Streaming Media
Files
Download Full Text (704 KB)
Course Code
MCB
Course Number
4920C
Faculty/Instructor
George Aranjuez
Faculty/Instructor Email
George.Aranjuez@ucf.edu
Abstract, Summary, or Creative Statement
Chlamydia trachomatis is a bacterial pathogen which utilizes secretion of effector proteins which target host cellular processes, while evading the host immune system. Using the pUAST vector and the GAL4/UAS expression system, it is possible to enhance the expression of any select Chlamydia protein effector. We took steps to purify one of these effectors, CTL0398, through PCR amplification, Gel Electrophoresis, and Colony PCR. These procedures were done with the hopes of future studies uncovering the true function of the select effector, and if there is any manifested phenotypic change in drosophila.
Keywords
Drosophila; Chlamydia; trachomatis
Recommended Citation
Wagner, Zachary E. and Garcia, Kenneth, "Purification and Application of Chlamydia trachomatis Effector CTL0398" (2025). High Impact Practices Student Showcase Spring 2025. 15.
https://stars.library.ucf.edu/hip-2025spring/15
