Development of a panel of y-chromosome markers for forensic use

Keywords

Forensic sciences, Y chromosome

Abstract

he aim of the present work was to improve the discriminatory potentia􀀋 and hence the probative value, ofY-STR based testing by extending the set ofY chromosome STR loci available for operational use. In accord with the requirements of a multiplex system developed for forensic use, also important was the maximiz.ation the number of loci that can be co-amplified, ensuring appropriate assay sensitivity (1-3 ng of input genomic DNA), balancing inter-locus signals and minimizing confounding female DNA artifacts. Two Y chromosome STR systems, multiplex I (MP I) and multiplex II (MP II), have been developed. These allow for the robust co-amplification of 18 Y STRs and their subsequent separation and detection using a standard capillary electrophoresis analytical platform. The two multiplex systems are robust over a wide range of primer, magnesium and DNA polymerase concentrations and perform well under a variety of cycling conditions. Complete male haplotypes can be obtained with as little as 100-250 pg of template DNA. MPI and MPII have been optimized such that few confounding female DNA artifacts are obtained at a female:male DNA ratio of 100:1. Thus it is often unnecessary to employ a differential extraction strategy to obtain a male haplotype ( or haplotypes in the case of multiple male donors) in cases of sexual assault.

Notes

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Graduation Date

2001

Advisor

Ballantyne, Jack

Degree

Master of Science (M.S.)

College

College of Arts and Sciences

Department

Chemistry

Format

PDF

Pages

104 p.

Language

English

Length of Campus-only Access

None

Access Status

Masters Thesis (Open Access)

Identifier

DP0028715

Subjects

Arts and Sciences -- Dissertations, Academic; Dissertations, Academic -- Arts and Sciences

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