Dna Antenna Tile-Associated Deoxyribozyme Sensor With Improved Sensitivity

Keywords

deoxyribozymes; diffusion-limited reactions; DNA crossover tile; mycobacterium tuberculosis; split hybridization probes

Abstract

Some natural enzymes increase the rate of diffusion-limited reactions by facilitating substrate flow to their active sites. Inspired by this natural phenomenon, we developed a strategy for efficient substrate delivery to a deoxyribozyme (DZ) catalytic sensor. This resulted in a three- to fourfold increase in sensitivity and up to a ninefold improvement in the detection limit. The reported strategy can be used to enhance catalytic efficiency of diffusion-limited enzymes and to improve sensitivity of enzyme-based biosensors.

Publication Date

11-3-2016

Publication Title

ChemBioChem

Volume

17

Issue

21

Number of Pages

2038-2041

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1002/cbic.201600438

Socpus ID

84992491297 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/84992491297

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