Title

Characterization of the IFN-gamma T-cell responses to immediate early antigens in humans with genital herpes

Authors

Authors

R. P. Braun; L. G. Payne;L. Dong

Comments

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Abbreviated Journal Title

Virol. J.

Keywords

SIMPLEX-VIRUS TYPE-1; GLYCOPROTEIN-D; LYMPHOCYTE CYTOTOXICITY; HSV-2; INFECTION; SENSORY NEURONS; VACCINE; LATENCY; REACTIVATION; LESIONS; EPIDEMIOLOGY; Virology

Abstract

Background: The IFN-gamma ELISPOT assay has been used to examine the T-cell repertoire for many disease states in humans but, as yet, not genital herpes. Using overlapping synthetic peptide libraries, an IFN-gamma ELISPOT assay was established that could measure CD4 and CD8 T-cell responses to HSV-2 antigens in patients with genital herpes. Results: In unexpanded T-cells isolated from peripheral blood, CD4 responses were readily measured against four immediate early antigens (ICP0, ICP4, ICP22 and ICP27), VP22 and gD. The CD4 responses were characterized by a low number of positive cells which produced large ELISPOTs. CD4 responses had a broad specificity and within individual patients several of the test antigens were recognized. In contrast, CD8 responses were found only in approximately 50% of patients and were typically specific to a single antigen. When disease status and immune responses were compared, an enhanced CD4 response to ICP4 in patients with a low recurrence rate was found. The ICP4 response was striking in three HSV-1 single positive genital herpes patients. Conclusion: The survey of T-cell responses is an important step to understand the host cellular immune response in individuals with genital herpes. The assay described here has the capability of measuring CD4 and CD8 T-cell responses that may be used to correlate disease status with specific immune responses. In an evaluation of 18 subjects a trend of positive responses to an immediate early protein, ICP4, was found in individuals that had a low rate of disease recurrence.

Journal Title

Virology Journal

Volume

3

Publication Date

1-1-2006

Document Type

Article

Language

English

First Page

15

WOS Identifier

WOS:000247503500001

ISSN

1743-422X

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