Title

Artificial neural networks for qualitative and quantitative analysis of target proteins with polymerized liposome vesicles

Authors

Authors

M. Santos; S. Nadi; H. C. Goicoechea; M. K. HaIdar; A. D. Campiglia;S. Mallik

Comments

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Abbreviated Journal Title

Anal. Biochem.

Keywords

luminescence; lanthanide ions; polymerized liposomes; carbonic; anhydrase; gamma-globulins; human serum albumin; partial least squares; artificial neural network; PARTIAL LEAST-SQUARES; MULTIVARIATE CALIBRATION; CAPILLARY-ELECTROPHORESIS; LIPID-MEMBRANES; GAMMA-GLOBULIN; SERUM-ALBUMIN; BINDING; MICROARRAYS; REGRESSION; IONS; Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical

Abstract

We investigate the feasibility of using the luminescence response of polymerized liposomes incorporating ethylenediaminetetraacetate europium(III) (EDTA-Eu3+) for monitoring protein concentrations in aqueous media. Quantitative analysis is based on the linear relationship between the luminescence enhancement of the lanthanide ion and protein concentration. Analytical figures of merit are presented for carbonic anhydrase, human serum albumin, gamma-globulins, and thermolysin. Qualitative analysis is based on the luminescence lifetime of the liposome sensor. This parameter, which follows well-behaved single exponential decays and provides characteristic values for each of the four studied proteins, demonstrates the selective potential for protein identification. Then partial least squares-1 and artificial neural networks are compared toward the quantitative and qualitative analysis of human serum albumin and carbonic anhydrase in binary mixtures without previous separation at the concentration levels found in aqueous humor. (c) 2006 Published by Elsevier Inc.

Journal Title

Analytical Biochemistry

Volume

361

Issue/Number

1

Publication Date

1-1-2007

Document Type

Article

Language

English

First Page

109

Last Page

119

WOS Identifier

WOS:000243572300012

ISSN

0003-2697

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