Oral delivery of human biopharmaceuticals, autoantigens and vaccine antigens bioencapsulated in plant cells
Abbreviated Journal Title
Int. J. Photoenergy
Autoantigens; Bioencapsulation; Diabetes; Hemophilia; Infectious; diseases; Lyophilization; Molecular farming; Oral vaccines; TOXIN-B-SUBUNIT; MEROZOITE SURFACE PROTEIN-1; APICAL MEMBRANE ANTIGEN-1; HEAT-LABILE ENTEROTOXIN; CHOLERA-TOXIN; TRANSGENIC CHLOROPLASTS; ESCHERICHIA-COLI; YERSINIA-PESTIS; FUSION PROTEIN; DRUG-DELIVERY; Pharmacology & Pharmacy
Among 12 billion injections administered annually, unsafe delivery leads to >20 million infections and >100 million reactions. In an emerging new concept, freeze-dried plant cells (lettuce) expressing vaccine antigens/biopharmaceuticals are protected in the stomach from acids/enzymes but are released to the immune or blood circulatory system when plant cell walls are digested by microbes that colonize the gut. Vaccine antigens bioencapsulated in plant cells upon oral delivery after priming, conferred both mucosal and systemic immunity and protection against bacterial, viral or protozoan pathogens or toxin challenge. Oral delivery of autoantigens was effective against complications of type 1 diabetes and hemophilia, by developing tolerance. Oral delivery of proinsulin or exendin-4 expressed in plant cells regulated blood glucose levels similar to injections. Therefore, this new platform offers a low cost alternative to deliver different therapeutic proteins to combat infectious or inherited diseases by eliminating inactivated pathogens, expensive purification, cold storage/transportation and sterile injections. (C) 2012 Elsevier B.V. All rights reserved.
Advanced Drug Delivery Reviews
Adv. Drug Deliv. Rev.
"Oral delivery of human biopharmaceuticals, autoantigens and vaccine antigens bioencapsulated in plant cells" (2013). Faculty Bibliography 2010s. 4252.