Site-Specific Structural Variations Accompanying Tubular Assembly of the HIV-1 Capsid Protein
Abbreviated Journal Title
J. Mol. Biol.
human immunodeficiency virus; AIDS; solid-state NMR; electron; microscopy; automated resonance assignment; SOLID-STATE NMR; NUCLEAR-MAGNETIC-RESONANCE; TERMINAL DIMERIZATION; DOMAIN; ANGLE-SPINNING NMR; 3-DIMENSIONAL STRUCTURE; MOLECULAR-STRUCTURE; CRYSTAL-STRUCTURE; AMYLOID FIBRILS; TORSION ANGLES; CYCLOPHILIN-A; Biochemistry & Molecular Biology
The 231-residue capsid (CA) protein of human immunodeficiency virus type 1 (HIV-1) spontaneously self-assembles into tubes with a hexagonal lattice that is believed to mimic the surface lattice of conical capsid cores within intact virions. We report the results of solid-state nuclear magnetic resonance (NMR) measurements on HIV-1 CA tubes that provide new information regarding changes in molecular structure that accompany CA self-assembly, local dynamics within CA tubes, and possible mechanisms for the generation of lattice curvature. This information is contained in site-specific assignments of signals in two- and three-dimensional solid-state NMR spectra, conformation-dependent N-15 and C-13 NMR chemical shifts, detection of highly dynamic residues under solution NMR conditions, measurements of local variations in transverse spin relaxation rates of amide H-1 nuclei, and quantitative measurements of site-specific N-15-N-15 dipole dipole couplings. Our data show that most of the CA sequence is conformationally ordered and relatively rigid in tubular assemblies and that structures of the N-terminal domain (NTD) and the C-terminal domain (CTD) observed in solution are largely retained. However, specific segments, including the N-terminal 13-hairpin, the cyclophilin A binding loop, the inter-domain linker, segments involved in intermolecular NTD CTD interactions, and the C-terminal tail, have substantial static or dynamical disorder in tubular assemblies. Other segments, including the 310-helical segment in CTD, undergo clear conformational changes. Structural variations associated with curvature of the CA lattice appear to be localized in the inter-domain linker and intermolecular NTD CTD interface, while structural variations within NTD hexamers, around local 3-fold symmetry axes, and in CTD CTD dimerization interfaces are less significant. Published by Elsevier Ltd.
Journal of Molecular Biology
"Site-Specific Structural Variations Accompanying Tubular Assembly of the HIV-1 Capsid Protein" (2014). Faculty Bibliography 2010s. 5043.