Chloroplast-derived vaccine antigens confer dual immunity against cholera and malaria by oral or injectable delivery
Abbreviated Journal Title
Plant Biotechnol. J.
cholera; malaria; chloroplast; vaccine; oral delivery; lettuce; TOXIN-B-SUBUNIT; HEAT-LABILE ENTEROTOXIN; TRANSGENIC TOBACCO; CHLOROPLASTS; OUTER-MEMBRANE VESICLES; NONOBESE DIABETIC MICE; PLANT-BASED VACCINE; LACTUCA-SATIVA L.; PLASMODIUM-FALCIPARUM; ESCHERICHIA-COLI; PROTECTIVE ANTIGEN; Biotechnology & Applied Microbiology; Plant Sciences
Cholera and malaria are major diseases causing high mortality The only licensed cholera vaccine is expensive, immunity is lost in children within 3 years and adults are not fully protected No vaccine is yet available for malaria Therefore, in this study, the cholera toxin-B subunit (CTB) of Vibrio cholerae fused to malarial vaccine antigens apical membrane antigen-1 (AMA1) and merozoite surface protein-1 (MSP1) was expressed in lettuce and tobacco chloroplasts Southern blot analysis confirmed homoplasmy and stable integration of transgenes CTB-AMA1 and CTB-MSP1 fusion proteins accumulated up to 13 17% and 10 11% (total soluble protein, TSP) in tobacco and up to 7 3% and 6 1 % (TSP) in lettuce, respectively Nine groups of mice (n = 10/group) were immunized subcutaneously (SQV) or orally (ORV) with purified antigens or transplastomic tobacco leaves Significant levels of antigen-specific antibody titres of immunized mice completely inhibited proliferation of the malarial parasite and cross-reacted with the native parasite proteins in immunoblots and immunofluorescence studies Protection against cholera toxin challenge in both ORV (100%) and SQV (89%) mice correlated with CTB-specific titres of intestinal, serum IgA and IgG1 in ORV and only IgG1 in SQV mice, but no other immunoglobu[in Increasing numbers of interleukin-10(+) T cell but not Foxp3(+) regulatory T cells, suppression of interferon-gamma and absence of interleukin-17 were observed in protected mice, Suggesting that immunity is conferred via the Th1/Th2 immune response Dual immunity against two major infectious diseases provided by chloroplast-derived vaccine antigens for long-term (>300 days, 50% Of mouse life span) offers a realistic platform for low cost vaccines and insight into mucosal and systemic immunity
Plant Biotechnology Journal
"Chloroplast-derived vaccine antigens confer dual immunity against cholera and malaria by oral or injectable delivery" (2010). Faculty Bibliography 2010s. 86.