A defined long-term in vitro tissue engineered model of neuromuscular junctions
Abbreviated Journal Title
In vitro test; Muscle; Nerve tissue engineering; Neural cell; Surface; modification; Self assembly; SERUM-FREE MEDIUM; AMYLOID PRECURSOR PROTEIN; SKELETAL-MUSCLE CELLS; SPINAL-CORD NEURONS; SILICON MICROSTRUCTURES; ORGANOSILANE SURFACE; GROWTH-FACTORS; DIFFERENTIATION; PROMOTES; CULTURE; Engineering, Biomedical; Materials Science, Biomaterials
Neuromuscular junction (NMJ) formation, occurring between motoneurons and skeletal muscle, is a complex multistep process involving a variety of signaling molecules and pathways. In vitro motoneuron-muscle co-cultures are powerful tools to study the role of different growth factors, hormones and cellular structures involved in NMJ formation. In this study, a serum-free culture system utilizing defined temporal growth factor application and a non-biological substrate resulted in the formation of robust NMJs. The system resulted in long-term survival of the co-culture and selective expression of neonatal myosin heavy chain, a marker of myotube maturation. NMJ formation was verified by colocalization of dense clusters of acetylcholine receptors visualized using alpha-bungarotoxin and synaptophysin containing vesicles present in motoneuron axonal terminals. This model will find applications in basic NMJ research and tissue engineering applications such as bio-hybrid device development for limb prosthesis and regenerative medicine as well as for high-throughput drug and toxin screening applications. (C) 2010 Elsevier Ltd. All rights reserved.
"A defined long-term in vitro tissue engineered model of neuromuscular junctions" (2010). Faculty Bibliography 2010s. 82.