Title

Fluorescent Dyes As Probes To Study Lipid-Binding Proteins

Keywords

ANS, bisANS; Binding; Fluorescence; Lipid-binding proteins

Abstract

We studied the equilibrium binding of two hydrophobic fluorescent dyes, ANS and bisANS, to four members of a family of intracellular lipid-binding proteins: IFABP, CRABP I, CRABP II, and ILBP. The spectral and binding parameters for the probes bound to the proteins were determined. Typically, there was a single binding site on each protein for the ligands. However, IFABP cooperatively bound a second bisANS molecule in the binding pocket. Comparative analysis of affinities and spectral characteristics for the two probes allowed us to examine the contributions of electrostatic and hydrophobic interactions to the binding process, and to address some aspects of the internal structure of the studied proteins. © 2003 Wiley-Liss, Inc.

Publication Date

11-15-2003

Publication Title

Proteins: Structure, Function and Genetics

Volume

53

Issue

3

Number of Pages

607-615

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1002/prot.10401

Socpus ID

0242330749 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/0242330749

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