Nanoparticle Delivery Of Curcumin Induces Cellular Hypoxia And Ros-Mediated Apoptosis: Via Modulation Of Bcl-2/Bax In Human Neuroblastoma

Abstract

In this study, several formulations of nanoceria and dextran-nanoceria with curcumin, each demonstrated to have anti-cancer properties, were synthesized and applied as treatment for human childhood neuroblastoma. The anti-cancer activities of these formulations were explored in neuroblastoma models of both MYCN-amplified and non-amplified cell lines. Ceria nanoparticles, coated with dextran and loaded with curcumin, were found to induce substantial cell death in neuroblastoma cells (up to a 2-fold and a 1.6-fold decrease in cell viability for MYCN-upregulated and normal expressing cell lines, respectively; ∗p < 0.05) while producing no or only minor toxicity in healthy cells (no toxicity at 100 μM; ∗∗p < 0.01). This formulation evokes prolonged oxidative stress, stabilizing HIF-1α, and inducing caspase-dependent apoptosis (up to a 2.4-fold increase over control; ∗p < 0.05). Overall, nano-therapeutic treatments showed a more pronounced effect in MYCN-amplified cells, which are traditionally more resistant to drug therapies. These results represent a very promising alternative to small molecule drug therapies for robust cancers.

Publication Date

8-7-2017

Publication Title

Nanoscale

Volume

9

Issue

29

Number of Pages

10375-10387

Document Type

Article

Personal Identifier

scopus

DOI Link

https://doi.org/10.1039/c7nr02770b

Socpus ID

85026481295 (Scopus)

Source API URL

https://api.elsevier.com/content/abstract/scopus_id/85026481295

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