Title

Increased bronchoalveolar lavage human beta-defensin type 2 in bronchiolitis obliterans syndrome after lung transplantation

Authors

Authors

D. J. Ross; A. M. Cole; D. Yoshioka; A. K. Park; J. A. Belperio; H. Laks; R. M. Strieter; J. P. Lynch; B. Kubak; A. Ardehali;T. Ganz

Comments

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Abbreviated Journal Title

Transplantation

Keywords

beta-defensin; lung transplantation; bronchiolitis obliterans; ANTIMICROBIAL PEPTIDES; HUMAN BETA-DEFENSIN-2; INNATE IMMUNITY; ANTIBIOTICS; EXPRESSION; EPITHELIA; HBD-2; Immunology; Surgery; Transplantation

Abstract

Human beta-defensin-2 (HBD)2 is an antimicrobial peptide that participates in the innate host immune defense. HBD2 is present in bronchoalveolar lavage (BAL) fluid during conditions associated with air-way inflammation but not in normal subjects. We measured HBD2 concentrations by semiquantitative Western analysis in BAL of prelung transplant patients (PRE) and postlung-transplant BAL associated with either "quiescent" histopathology (i.e., without acute cellular rejection or infection) (NORMAL POST) or with bronchiolitis obliterans syndrome (BOS). HBD2 levels were not different for PRE (n=9) versus NORMAL POST-transplant BAL specimens (n=22) (204 +/- 180 vs. 82 +/- 60 pg/mL; P=NS). The BAL HBD2 concentrations were significantly elevated, however, with BOS (n=8) (1,270 430 pg/mL; P<0.001). HBD2 has been previously shown to elicit an adaptive immune response by means of recruitment of immature CD34(+) dendritic cells and memory (CD4(+)/CD45RO(+)) T lymphocytes through interactions with their chemokine receptor, CCR6. Furthermore, HBD2 with CD14 in human tracheobronchial epithelium can complex with "toll-like receptors" to activate the nuclear factor (NF)-kappaB pathway and therefore promote cytokine gene expression. We therefore speculate that complex interactions between adaptive and innate immunity may contribute to the propagation of airway inflammation in BOS.

Journal Title

Transplantation

Volume

78

Issue/Number

8

Publication Date

1-1-2004

Document Type

Article

Language

English

First Page

1222

Last Page

1224

WOS Identifier

WOS:000225110500025

ISSN

0041-1337

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