Increased bronchoalveolar lavage human beta-defensin type 2 in bronchiolitis obliterans syndrome after lung transplantation

    Authors

    D. J. Ross; A. M. Cole; D. Yoshioka; A. K. Park; J. A. Belperio; H. Laks; R. M. Strieter; J. P. Lynch; B. Kubak; A. Ardehali;T. Ganz

    Comments

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    Abbreviated Journal Title

    Transplantation

    Keywords

    beta-defensin; lung transplantation; bronchiolitis obliterans; ANTIMICROBIAL PEPTIDES; HUMAN BETA-DEFENSIN-2; INNATE IMMUNITY; ANTIBIOTICS; EXPRESSION; EPITHELIA; HBD-2; Immunology; Surgery; Transplantation

    Abstract

    Human beta-defensin-2 (HBD)2 is an antimicrobial peptide that participates in the innate host immune defense. HBD2 is present in bronchoalveolar lavage (BAL) fluid during conditions associated with air-way inflammation but not in normal subjects. We measured HBD2 concentrations by semiquantitative Western analysis in BAL of prelung transplant patients (PRE) and postlung-transplant BAL associated with either "quiescent" histopathology (i.e., without acute cellular rejection or infection) (NORMAL POST) or with bronchiolitis obliterans syndrome (BOS). HBD2 levels were not different for PRE (n=9) versus NORMAL POST-transplant BAL specimens (n=22) (204 +/- 180 vs. 82 +/- 60 pg/mL; P=NS). The BAL HBD2 concentrations were significantly elevated, however, with BOS (n=8) (1,270 430 pg/mL; P < 0.001). HBD2 has been previously shown to elicit an adaptive immune response by means of recruitment of immature CD34(+) dendritic cells and memory (CD4(+)/CD45RO(+)) T lymphocytes through interactions with their chemokine receptor, CCR6. Furthermore, HBD2 with CD14 in human tracheobronchial epithelium can complex with "toll-like receptors" to activate the nuclear factor (NF)-kappaB pathway and therefore promote cytokine gene expression. We therefore speculate that complex interactions between adaptive and innate immunity may contribute to the propagation of airway inflammation in BOS.

    Journal Title

    Transplantation

    Volume

    78

    Issue/Number

    8

    Publication Date

    1-1-2004

    Document Type

    Article

    Language

    English

    First Page

    1222

    Last Page

    1224

    WOS Identifier

    WOS:000225110500025

    ISSN

    0041-1337

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