Abstract
The MS-818 compound is used in the proliferation process of neuronal cells and many biological activities that accompany this process such as astrocyte differentiation, inhibition of neuronal apoptosis, and fraction repairs. We do know the effects of this compound, but the mechanism of action remained uncertain until now. To determine the pathway of this compound, NT2 cells were cultured and lysed to isolate the proteins. Affinity Chromatography was performed in order to immobilize the MS-818 compound to a Hi-Trap NHS column. The NT2 protein sample was injected through the column and eluted with a MS-818 concentrated, high salt content elution buffer. SDS-PAGE was then performed to isolate the proteins that bound to MS-818. The gel was visualized using Coomassie Blue. The results indicate that there are two proteins associated in the mechanism of this compound. A standard protein marker ranging from 10 kDa to 250 kDa was used to compare the bands. The findings indicate that one of the protein bands is slightly less than 250kDa and the other is between 50-75 kDa. When the proteins are confirmed by mass spectrometry sequencing, this will help to promote this compound as a drug candidate.
Notes
If this is your Honors thesis, and want to learn how to access it or for more information about readership statistics, contact us at STARS@ucf.edu
Thesis Completion
2011
Semester
Summer
Advisor
Sugaya, Kiminobu
Degree
Bachelor of Science (B.S.)
College
College of Medicine
Degree Program
Molecular Biology and Microbiology
Subjects
Dissertations, Academic -- Medicine;Medicine -- Dissertations, Academic
Format
Identifier
CFH0004076
Language
English
Access Status
Open Access
Length of Campus-only Access
None
Document Type
Honors in the Major Thesis
Recommended Citation
Dass, Charlene Seraphina, "Stimulator of neurotropic effects determining the mechanism of action of the MS-818 compound through protein identification by affinity chromatography and SDS-PAGE" (2011). HIM 1990-2015. 1197.
https://stars.library.ucf.edu/honorstheses1990-2015/1197