Two-photon flourescence imaging is fast becoming the premier technique for nondestructive, 3-D imaging in the biological and biomedical research fields, both in living and nonliving systems. This technology currently used in two-photon flourescene imaging emanates from conventional flourescence microscopy.
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College of Sciences
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Nonprovisional Application Record
Belfield, Kevin and Schafer, Katherine, "Reactive Probes for Two Photon Fluorescent Imaging and sensing." (2007). UCF Patents. 489.