Abstract

The effect of adherent cells, prostaglandin E2 (PGE2), and indomethacin on LAK cell activity of C57BL/6J (B6) mice was investigated. Depletion of adherent cells from splenocyte cultures prior to LAK cell generation increased LAK cell cytotoxicity by 60% compared to non-depleted splenocyte cultures. Depletion of adherent cells from splenocyte cultures following five days of incubation and prior to the LAK cell assay resulted in a similar enhancement. Indomethacin, an inhibitor of prostaglandin synthesis, enhanced LAK cell cytotoxicity in splenocyte cultures. PGE2 inhibited LAK cell cytotoxicity of adherent cell depleted splenocytes 28% and 88% at l0-6M and 10-5M. In vivo treatment of mice with indomethacin increased LAK cell cytotoxicity two fold compared to ethanol treated controls. LAK cell cytotoxicity was greatest in splenocytes from indomethacin treated mice cultured with additional indomethacin at 10.0 ug/ml. This study describes methods which increase murine LAK cell cytotoxicity.

Notes

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Graduation Date

1987

Semester

Spring

Advisor

Longley, Ross E.

Degree

Master of Science (M.S.)

College

College of Arts and Sciences

Degree Program

Microbiology

Format

PDF

Pages

54 p.

Language

English

Rights

Public Domain

Length of Campus-only Access

None

Access Status

Masters Thesis (Open Access)

Identifier

DP0020553

Accessibility Status

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Microbiology Commons

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