Abstract
Huanglongbing (HLB), more commonly known as citrus greening disease (CGD), is an insect-borne bacterial disease affecting citrus crops worldwide. CGD symptoms can be visually seen throughout the plant, displaying yellowing veins, asymmetrical chlorosis and blotchy leaves, leading to underdeveloped fruit with green colored and stained seeds. The mottling discoloration has been linked to zinc deficiency in the plant, thus monitoring varying zinc levels is our main concern. Well established tools for plant analysis such as Atomic Absorption Spectroscopy (AAS), X-Ray Fluorescence, among other methods are costly, non-portable and destructive. Here, we have developed and characterized two solid contact micro-ion-selective electrodes (SC-µ-ISEs). Each electrode plays a vital role in providing a non-destructive method for monitoring free zinc content in the leaves of citrus plants as well as determining the role of nitrate at the surface of the leaves. The zinc and nitrate sensors are composed of Zinc Ionophore I (80 mmol/kg) and KTFPB (10 mmol/kg) with a plasticized PVC matrix, as well as Tridodecylmethylammonium Chloride (TDMACl) (5 mmol/kg) with a plasticized PVC matrix, respectively. The detection of zinc and nitrate was done separately and simultaneously. Additional plans for this project include simultaneous detection of zinc and nitrate in leaf surface profiles via in situ analysis.
Date Created
January 2018
STARS Citation
Armas, Stephanie; Autrey, Cody; Blanco Martinez, Enrique; Church, Jared; Santra, Swadeshmukul; Hyoung Lee, Woo; and Chumbimuni-Torres, Karin, "Solid-Contact Micro-Ion-Selective Electrode for Analysis of Zinc in Plants" (2018). EGS Content. 28.
https://stars.library.ucf.edu/egs_content/28
https://works.bepress.com/cody-autrey/2/download/