Title

Isolation And Characterization Of Cross-Reactive Allergenic Components In Callistemon-Citrinis And Melaleuca-Quinquenervia Pollen By Trans-Blot Enzyme-Linked Crossed Immunoelectrophoresis

Comments

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Keywords

Allergy; Immunology

Abstract

Antigenic extracts obtained from Callistemon citrinis (bottlebrush) and Melaleuca quinquenervia (melaleuca) pollen were analyzed by crossed immunoelectrophoresis (CIE), tandem-crossed immunoelectrophoresis (TCIE), and trans-blot enzyme-linked crossed immunoelectrophoresis (TECIE). CIE analysis detected 14 and 12 antigenic components in bottlebrush and melaleuca, respectively. TCIE analysis of bottlebrush pollen, employing rabbit anti-melaleuca serum in the intermediate gel, demonstrated that all of the antigenic components detected were cross-reactive. Similar analysis of melaleuca using rabbit anti-bottlebrush serum in the intermediate gel also showed that all of the antigenic components were cross-reactive. At least three bottlebrush and two melaleuca cross-reactive pollen components were demonstrated to be allergenic by TECIE analysis of CIE and TCIE gels.

Journal Title

International Archives of Allergy and Applied Immunology

Volume

80

Issue/Number

3

Publication Date

1-1-1986

Document Type

Article

Language

English

First Page

278

Last Page

284

WOS Identifier

WOS:A1986D073100010

ISSN

0020-5915

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