Tissue Distribution Of Isocitrate Dehydrogenase In Drosophila-Melanogaster
Biochemistry & Molecular Biology; Entomology
Distribution of the soluble form of isocitrate dehydrogenase (ICDH) in imaginal discs, some larval and some adult Drosophila melanogaster structures was determined. ICDH distribution in imaginal discs provides an opportunity to study in morphologically similar cells the degree of biochemical differentiation, the ontogeny of ICDH pattern formation, tissue determination at the disc level and in some instances the position of developmental compartments. Differential activity for ICDH was observed in the eye-antennal disc, dorsal prothoracic disc, all leg discs, wing and haltere discs, genital disc, larval gut, plus the adult ovaries and the male genital structures. Uniform activity was found in the labial disc, larval salivary gland cells, nests of abdominal histoblasts and in larval fat bodies. No detectable ICDH activity was observed in the imaginal cells of the clypeus-labrum, the imaginal cells of the foregut, the imaginal ring of the hindgut, or in clusters of imaginal cells of ICDH negative regions of the midgut. It is concluded that ICDH, like aldehyde oxidase, is non-random in its tissue distribution and does provide an additional internal histochemical marker for developmental studies.
Cunningham, Glenn N. and Kuhn, David T., "Tissue Distribution Of Isocitrate Dehydrogenase In Drosophila-Melanogaster" (1980). Faculty Bibliography 1980s. 55.