Title

Allergenic Cross-Reactivity Between Callistemon-Citrinis And Melaleuca-Quinquenervia Pollens

Comments

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Abbreviated Journal Title

IEEE Trans. Antennas Propag.

Keywords

Allergy; Immunology

Abstract

Aqueous extracts of both Callistemon citrinis (bottlebrush) and Melaleuca quinquenervia (melaleuca) were analyzed for allergenic cross-reactivity. Inhibition analysis using the radioallergosorbent test (RAST) was performed on the ammonium bicarbonate extracts of bottlebrush (NH4B) and melaleuca (NH4M) pollens. RAST inhibition analysis demonstrated that the extracts contained allergenically cross-reactive components. Sephadex G-100 column chromatography of NH4B and NH4M extracts resulted in at least 4 distinct peaks for each extract analyzed. These fractions were designated NH4B1-NH4B4 and NH4M1-NH4M4. A modified dot-blot assay for detection of allergenic components was utilized to show that the first elution peaks of bottlebrush and melaleuca, NH4B1 and NH4M1, respectively, contained allergenic components. These allergenic components, NH4B1 and NH4M1, had estimated molecular weights of 50,000 and 35,000 daltons, respectively.

Journal Title

IEEE Transactions on Antennas and Propagation

Volume

86

Issue/Number

1

Publication Date

1-1-1988

Document Type

Article

Language

English

First Page

35

Last Page

41

WOS Identifier

WOS:A1988N567400005

ISSN

0020-5915

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