Field Detection Of Citrus Blight Using Immunological Techniques

    Authors

    E. Blakely;A. Poling

    Comments

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    Abbreviated Journal Title

    Plant Dis.

    Keywords

    VALENCIA ORANGE TREES; POLYACRYLAMIDE GELS; SENSITIVE METHOD; PROTEINS; WATER; HEALTHY; TISSUES; Plant Sciences

    Abstract

    Eighty-six sweet orange trees from three Florida grove locations were diagnosed as being healthy or blighted using a western blot system with proteins extracted from citrus leaves. Extracted proteins were separated on 18% SDS-PAGE gels, electroblotted onto polyvinylidenedifluoride membrane, and probed with citrus blight antisera obtained from blighted citrus trees. This blot system was as reliable as conventional diagnostic systems and in several cases detected the presence of blight before increased tissue concentrations of zinc, decreased water uptake, and wilt were observed. Results were the same for samples taken during winter dormant periods and samples taken during spring growth. The use of leaf samples for the diagnosis of citrus blight has the advantage of allowing repeated sampling, unlike xylem zinc analysis and water injection techniques.

    Journal Title

    Plant Disease

    Volume

    75

    Issue/Number

    5

    Publication Date

    1-1-1991

    Document Type

    Article

    Language

    English

    First Page

    447

    Last Page

    450

    WOS Identifier

    WOS:A1991FH46200003

    ISSN

    0191-2917

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