Application of X-ray micrography and imaging to study the effect of gentamicin on Pseudomonas aeruginosa

    Authors

    J. M. Rajyaguru; D. S. Torres; E. Abel; M. C. Richardson;M. J. Muszynski

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    J. Antimicrob. Chemother.

    Keywords

    CELL-ENVELOPE; Infectious Diseases; Microbiology; Pharmacology & Pharmacy

    Abstract

    Aminoglycosides disrupt the outer membrane of Pseudomonas aeruginosa to facilitate access to their intracellular target. High-resolution X-ray micrography of live specimens is a relatively new technique. We used laser (nanosecond) plasma to image live cells of P. aeruginosa ATCC 27853. After exposure to 25 mg/L gentamicin for 15 min. we observed perturbation of the cell surface, membrane blebbings (370 nm and 273 nm diameter) away from the sell, formation of distinct channels (241 nm long) resulting from indentation and induction of cell elongation from 3-3.6 mu m (control) to 4.6-5.26 mu m (gentamicin-treated cells). These data illustrate the potential of high-resolution X-ray micrography for studying effects of drugs on live microbiological specimens.

    Journal Title

    Journal of Antimicrobial Chemotherapy

    Volume

    41

    Issue/Number

    5

    Publication Date

    1-1-1998

    Document Type

    Article

    Language

    English

    First Page

    557

    Last Page

    561

    WOS Identifier

    WOS:000074481700007

    ISSN

    0305-7453

    Share

    COinS