Title

Effects of nucleoside analog incorporation on DNA binding to the DNA binding domain of the GATA-1 erythroid transcription factor

Authors

Authors

M. Foti; J. G. Omichinski; S. Stahl; D. Maloney; J. West;B. I. Schweitzer

Comments

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Abbreviated Journal Title

FEBS Lett.

Keywords

ganciclovir; cytosine arabinoside; GATA-1; binding; nuclear magnetic; resonance; THYMIDINE KINASE GENE; RESTRAINED MOLECULAR-DYNAMICS; RELAXATION MATRIX; REFINEMENT; BRAIN-TUMORS; IN-VIVO; 9-(1, 3-DIHYDROXY-2-PROPOXYMETHYL)GUANINE TRIPHOSPHATE; 1-BETA-D-ARABINOFURANOSYLCYTOSINE 5'-TRIPHOSPHATE; TRANSPLANT; RECIPIENTS; COLONY FORMATION; NUCLEIC-ACIDS; Biochemistry & Molecular Biology; Biophysics; Cell Biology

Abstract

We investigate here the effects of the incorporation of the nucleoside analogs araC (1-beta-D-arabinofuranosylcytosine) and ganciclovir (9-[(1,3-dihydroxy-2-propoxy)methyl] guanine) into the DNA binding recognition sequence for the GATA-1 erythroid transcription factor. A 10-fold decrease in binding affinity was observed for the ganciclovir-substituted DNA complex in comparison to an unmodified DNA of the same sequence composition. AraC substitution did not result in any changes in binding affinity, H-1-N-15 HSQC and NOESY NMR experiments revealed a number of chemical shift changes in both DNA and protein in the ganciclovir-modified DNA-protein complex when compared to the unmodified DNA-protein complex, These changes in chemical shift and binding affinity suggest a change in the binding mode of the complex when ganciclovir is incorporated into the GATA DNA binding site. (C) 1999 Federation of European Biochemical Societies.

Journal Title

Febs Letters

Volume

444

Issue/Number

1

Publication Date

1-1-1999

Document Type

Article

Language

English

First Page

47

Last Page

53

WOS Identifier

WOS:000078616200010

ISSN

0014-5793

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