Direct ultrastructural imaging of macrophages using a novel X-ray contact microscopy

    Authors

    P. Kado; H. Richardson; J. M. Rajyaguru; M. J. Muszynski; H. Friedman;Y. Yamamoto

    Comments

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    Abbreviated Journal Title

    Proc. Soc. Exp. Biol. Med.

    Keywords

    LEGIONELLA-PNEUMOPHILA; LASER; RESOLUTION; SPERM; Medicine, Research & Experimental

    Abstract

    A compact, high-resolution, laser-plasma, x-ray contact microscopy method using a table-top Nd:glass laser system has been developed, This x-ray microscopy system was applied for the observation of macrophage ultrastructures, These images were produced using proximity imaging in which a 5-ns pulse of soft x-rays with wavelengths near and inside the water windows (23 Angstrom-44 Angstrom) produced by the laser-plasma were absorbed by the specimen and then registered on a photo resist. The x-ray images imprinted on the photo resist were then developed and analyzed with an atomic force microscope (AFM). Mouse thioglycollate-elicited peritoneal macrophages in suspension were examined by this new x-ray microscope. The x-ray images of the macrophages were compared with those observed by conventional transmission electron microscopy (TEM). The x-ray images showed no obvious organelles, Including the nucleus and endoplasmic reticulum, as can be seen with TEM, but high- and low-contrast structures caused by mass distribution of carbon were observed. Thus, using the x-ray microscopy we visualized the first x-ray images of macrophage ultrastructures. The successful x-ray imaging of macrophage ultrastructure indicates that proximity x-ray microscopy may be of value in studying physiology linked to the dynamics of a cell.

    Journal Title

    Proceedings of the Society for Experimental Biology and Medicine

    Volume

    220

    Issue/Number

    1

    Publication Date

    1-1-1999

    Document Type

    Article

    Language

    English

    First Page

    27

    Last Page

    30

    WOS Identifier

    WOS:000077911100004

    ISSN

    0037-9727

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