Direct ultrastructural imaging of macrophages using a novel X-ray contact microscopy
Abbreviated Journal Title
Proc. Soc. Exp. Biol. Med.
LEGIONELLA-PNEUMOPHILA; LASER; RESOLUTION; SPERM; Medicine, Research & Experimental
A compact, high-resolution, laser-plasma, x-ray contact microscopy method using a table-top Nd:glass laser system has been developed, This x-ray microscopy system was applied for the observation of macrophage ultrastructures, These images were produced using proximity imaging in which a 5-ns pulse of soft x-rays with wavelengths near and inside the water windows (23 Angstrom-44 Angstrom) produced by the laser-plasma were absorbed by the specimen and then registered on a photo resist. The x-ray images imprinted on the photo resist were then developed and analyzed with an atomic force microscope (AFM). Mouse thioglycollate-elicited peritoneal macrophages in suspension were examined by this new x-ray microscope. The x-ray images of the macrophages were compared with those observed by conventional transmission electron microscopy (TEM). The x-ray images showed no obvious organelles, Including the nucleus and endoplasmic reticulum, as can be seen with TEM, but high- and low-contrast structures caused by mass distribution of carbon were observed. Thus, using the x-ray microscopy we visualized the first x-ray images of macrophage ultrastructures. The successful x-ray imaging of macrophage ultrastructure indicates that proximity x-ray microscopy may be of value in studying physiology linked to the dynamics of a cell.
Proceedings of the Society for Experimental Biology and Medicine
"Direct ultrastructural imaging of macrophages using a novel X-ray contact microscopy" (1999). Faculty Bibliography 1990s. 2689.