Occurrence of the IS900 gene in Mycobacterium avium complex derived from HIV patients

    Authors

    S. A. Naser; J. Felix; H. Liping; C. Romero; N. Naser; A. Walsh;W. Safranek

    Comments

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    Abbreviated Journal Title

    Mol. Cell. Probes

    Keywords

    IS900; mycobacteria; HIV; Crohn's disease; PCR; POLYMERASE CHAIN-REACTION; INSERTION ELEMENT; CROHNS-DISEASE; SUBSP; SILVATICUM; CONTROL TISSUES; JOHNES-DISEASE; PARATUBERCULOSIS; IDENTIFICATION; TUBERCULOSIS; FRAGMENT; Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Cell Biology

    Abstract

    The occurrence of the insertion sequence IS900 in Mycobacterium avium subsp. avium strains isolated from HIV infected patients has been investigated. In this study, genomic DNA from 62 mycobacterial isolates [31 strains of M. avium complex (MAC) consisting of 26 M. avium subsp. avium HIV-isolates and five non-HIV isolates and 31 additional Mycobacterium species] were analysed by an IS900-based polymerase chain reaction (PCR) assay and Southern hybridization using a non-radioactive-labelled 251 bp DNA fragment located at the 5'-region of the IS900 sequence. As expected, none of the 28 Mycobacterium species contained the IS900 in their genomic DNA. Of the 26 M, avium subsp. avium HIV-isolates, 15 (57.6%) were strongly positive for the IS900 or IS900 related sequence. The five pulmonary non-HIV MAC isolates were negative for the IS900. As expected, the three strains of M. avium subsp. paratuberculosis were positive. This PCR guided study suggest that the IS900 gene is very common in clinical strains of M. avium subsp. avium especially those isolated from HIV patients. Ultimately the IS900 PCR-based assay may provide a useful tool for diagnostic and epidemiological studies related to MAC infections in HIV patients. (C) 1999 Academic Press.

    Journal Title

    Molecular and Cellular Probes

    Volume

    13

    Issue/Number

    5

    Publication Date

    1-1-1999

    Document Type

    Article

    Language

    English

    First Page

    367

    Last Page

    372

    WOS Identifier

    WOS:000083117500005

    ISSN

    0890-8508

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