Title

Direct Imaging In A Water Layer Of Human-Chromosome Fibers Composed Of Nucleosomes And Their Higher-Order Structures By Laser-Plasma X-Ray Contact Microscopy

Authors

Authors

Y. Kinjo; K. Shinohara; A. Ito; H. Nakano; M. Watanabe; Y. Horiike; Y. Kikuchi; M. C. Richardson;K. A. Tanaka

Comments

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Abbreviated Journal Title

J. Microsc.-Oxf.

Keywords

LASER-PLASMA X-RAYS; CONTACT MICROSCOPY; HYDRATED HUMAN CHROMOSOME; FIBER; UNFIXED; UNSTAINED; NUCLEOSOME; SUPERBEAD; ORGANIZATION; CHROMATIN; Microscopy

Abstract

X-ray contact microscopy with a 300-ps-duration laser-plasma X-ray source has been used to image hydrated human chromosomes. Clearly imaged are individual nucleosomes and their higher-order particles (superbeads), elementary chromatin fibrils c. 30nm in diameter and their higher-order fibres of various sizes up to c. 120nm in diameter. The results demonstrate that X-ray microscopy is now capable of opening a new path of investigation into the detailed structures of hydrated chromosome fibres in their natural state.

Journal Title

Journal of Microscopy-Oxford

Volume

176

Publication Date

1-1-1994

Document Type

Article

Language

English

First Page

63

Last Page

74

WOS Identifier

WOS:A1994PR98300008

ISSN

0022-2720

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