Direct Imaging In A Water Layer Of Human-Chromosome Fibers Composed Of Nucleosomes And Their Higher-Order Structures By Laser-Plasma X-Ray Contact Microscopy

    Authors

    Y. Kinjo; K. Shinohara; A. Ito; H. Nakano; M. Watanabe; Y. Horiike; Y. Kikuchi; M. C. Richardson;K. A. Tanaka

    Comments

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    Abbreviated Journal Title

    J. Microsc.-Oxf.

    Keywords

    LASER-PLASMA X-RAYS; CONTACT MICROSCOPY; HYDRATED HUMAN CHROMOSOME; FIBER; UNFIXED; UNSTAINED; NUCLEOSOME; SUPERBEAD; ORGANIZATION; CHROMATIN; Microscopy

    Abstract

    X-ray contact microscopy with a 300-ps-duration laser-plasma X-ray source has been used to image hydrated human chromosomes. Clearly imaged are individual nucleosomes and their higher-order particles (superbeads), elementary chromatin fibrils c. 30nm in diameter and their higher-order fibres of various sizes up to c. 120nm in diameter. The results demonstrate that X-ray microscopy is now capable of opening a new path of investigation into the detailed structures of hydrated chromosome fibres in their natural state.

    Journal Title

    Journal of Microscopy-Oxford

    Volume

    176

    Publication Date

    1-1-1994

    Document Type

    Article

    Language

    English

    First Page

    63

    Last Page

    74

    WOS Identifier

    WOS:A1994PR98300008

    ISSN

    0022-2720

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