Immunodetection And Comparison Of Melaleuca, Bottlebrush, And Bahia Pollens

    Authors

    M. Sweeney; S. Hosseiny; S. Hunter; S. D. Klotz; R. N. Gennaro;R. S. White

    Comments

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    Abbreviated Journal Title

    Int. Arch. Allergy Immunol.

    Keywords

    ALLERGEN CHARACTERIZATION; ALLERGENIC CROSS-REACTIVITY; CALLISTEMON; CITRINIS; IMMUNOBLOTTING; MELALEUCA LEUCADENDRON; PASPALUM NOTATUM; ALLERGENIC CROSS-REACTIVITY; ELECTROPHORETIC TRANSFER; CALLISTEMON-CITRINIS; POLYACRYLAMIDE GELS; BINDING PROTEINS; IGE; HYPERSENSITIVITY; IDENTIFICATION; ANTIBODIES; BLOT; Allergy; Immunology

    Abstract

    Pollen extracts of two trees, Callistemon citrinis (bottlebrush) and Melaleuca leucadendron (melaleuca), as well as the grass Paspalum notatum (bahia) were analyzed for antigenic and allergenic cross-reactivity using SDS-PAGE and Western blotting. SDS-PAGE analysis of all three pollen extracts revealed multiple antigenic components which were reactive with rabbit antisera made to each pollen extract. Comparison of reduced and nonreduced mobility patterns suggested the possible presence of multichain proteins linked by disulfide bonds. Clinical studies demonstrated that 81% of the patients skin test positive to at least one of the pollens were also positive to the other two. Sixty-three percent of allergic individuals studied showed a high correlation between skin test results and the number of IgE-binding components analyzed by immunoblotting. These IgE-reactive components were detected in the molecular weight range of 29-66 kD. Western blot analysis detected more IgE-binding components in bahia pollen extracts as compared with the tree pollens, consistent with other reports of grass pollens being more allergenic than those from trees and weeds. Each patient's serum had a unique IgE-binding pattern, indicating heterogeneity of immune response; however, common major determinants were detected by a large percentage of the allergic patient's sera.

    Journal Title

    International Archives of Allergy and Immunology

    Volume

    105

    Issue/Number

    3

    Publication Date

    1-1-1994

    Document Type

    Article

    Language

    English

    First Page

    289

    Last Page

    296

    WOS Identifier

    WOS:A1994PP37500013

    ISSN

    1018-2438

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