Current Status Of The Plasmodium Falciparum Genome Project

    Authors

    J. B. Dame; D. E. Arnot; P. F. Bourke; D. Chakrabarti; Z. Christodoulou; R. L. Coppel; A. F. Cowman; A. G. Craig; K. Fischer; J. Foster; N. Goodman; K. Hinterberg; A. A. Holder; D. C. Holt; D. J. Kemp; M. Lanzer; A. Lim; C. I. Newbold; J. V. Ravetch; G. R. Reddy; J. Rubio; S. M. Schuster; X. Z. Su; J. K. Thompson; F. Vital; T. E. Wellems;E. B. Werner

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    Abbreviated Journal Title

    Mol. Biochem. Parasitol.

    Keywords

    genome project; malaria; mapping; expressed sequence tag; Plasmodium; falciparum; review; yeast artificial chromosome; mung bean nuclease; cDNA; protozoa; EXPRESSED SEQUENCE TAGS; YEAST ARTIFICIAL CHROMOSOMES; MUNG BEAN; NUCLEASE; MULTIDRUG RESISTANCE GENE; RICH PROTEIN GENE; P-FALCIPARUM; CHLOROQUINE-RESISTANCE; CAENORHABDITIS-ELEGANS; ASPARTIC PROTEINASE; MALARIA PARASITES; Biochemistry & Molecular Biology; Parasitology

    Abstract

    The Plasmodium falciparum Genome Project is a collaborative effort by many laboratories that will provide detailed molecular information about the parasite, which may be used for developing practical control measures. Initial goals are to prepare an electronically indexed clone bank containing partially sequenced clones representing up to 80% of the parasite's genes and to prepare an ordered set of overlapping clones spanning each of the parasite's 14 chromosomes. Currently, clones of genomic DNA, prepared as yeast artificial chromosomes, are arranged into contigs covering similar to 70% of the genome of parasite clone 3D7, gene sequence tags are available from more than contigs covering similar to 70% of the genome of parasite clone 3D7, gene sequence tags are available from more than 20% of the parasite's genes, and similar to 5% of the parasite's genes are tentatively identified from similarity searches of entries in the international sequence databases. A total of > 0.5 Mb of P. falciparum sequence tag data is available. The gene sequence tags are presently being used to complete YAC contig assembly and localize the cloned genes to positions on the physical map in preparation for sequencing the genome. Routes of access to project information and services are described.

    Journal Title

    Molecular and Biochemical Parasitology

    Volume

    79

    Issue/Number

    1

    Publication Date

    1-1-1996

    Document Type

    Review

    Language

    English

    First Page

    1

    Last Page

    12

    WOS Identifier

    WOS:A1996VA64500001

    ISSN

    0166-6851

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