Title

Assessing mitochondrial morphology and dynamics using fluorescence wide-field microscopy and 3D image processing

Authors

Authors

W. Song; B. Bossy; O. J. Martin; A. Hicks; S. Lubitz; A. B. Knott;E. Bossy-Wetzel

Comments

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Abstract

Mitochondrial morphology and length change during fission and fusion and mitochondrial movement varies dependent upon the cell type and the physiological conditions. Here, we describe fundamental wide-field fluorescence microcopy and 3D imaging techniques to assess mitochondrial shape, number and length in various cell types including cancer cell lines, motor neurons and astrocytes. Furthermore, we illustrate how to assess mitochondrial fission and fusion events by 3D time-lapse imaging and to calculate mitochondrial length and numbers as a function of time. These imaging methods provide useful tools to investigate mitochondrial dynamics in health, aging and disease. (C) 2008 Elsevier Inc. All rights reserved.

Journal Title

Methods

Volume

46

Issue/Number

4

Publication Date

1-1-2008

Document Type

Article

First Page

295

Last Page

303

WOS Identifier

WOS:000261647200010

ISSN

1046-2023

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