Assessing mitochondrial morphology and dynamics using fluorescence wide-field microscopy and 3D image processing

    Authors

    W. Song; B. Bossy; O. J. Martin; A. Hicks; S. Lubitz; A. B. Knott;E. Bossy-Wetzel

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abstract

    Mitochondrial morphology and length change during fission and fusion and mitochondrial movement varies dependent upon the cell type and the physiological conditions. Here, we describe fundamental wide-field fluorescence microcopy and 3D imaging techniques to assess mitochondrial shape, number and length in various cell types including cancer cell lines, motor neurons and astrocytes. Furthermore, we illustrate how to assess mitochondrial fission and fusion events by 3D time-lapse imaging and to calculate mitochondrial length and numbers as a function of time. These imaging methods provide useful tools to investigate mitochondrial dynamics in health, aging and disease. (C) 2008 Elsevier Inc. All rights reserved.

    Journal Title

    Methods

    Volume

    46

    Issue/Number

    4

    Publication Date

    1-1-2008

    Document Type

    Article

    First Page

    295

    Last Page

    303

    WOS Identifier

    WOS:000261647200010

    ISSN

    1046-2023

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