Transcriptional analysis of diverse strains Mycobacterium avium subspecies paratuberculosis in primary bovine monocyte derived macrophages

    Authors

    X. C. Zhu; Z. J. Tu; P. M. Coussens; V. Kapur; H. Janagama; S. Naser;S. Sreevatsan

    Comments

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    Abbreviated Journal Title

    Microbes Infect.

    Keywords

    Mycobacterium avium subspecies paratuberculosis; SCOTS; Gene expression; macrophage; Virulence; TRANSCRIBED SEQUENCES SCOTS; FRAGMENT-LENGTH-POLYMORPHISM; COMPLETE; GENOME SEQUENCE; BLOOD MONONUCLEAR-CELLS; CROHNS-DISEASE PATIENTS; SELECTIVE CAPTURE; MOLECULAR EPIDEMIOLOGY; PHAGOSOMAL MATURATION; TUBERCULOSIS; IDENTIFICATION; Immunology; Infectious Diseases; Microbiology

    Abstract

    In this study we analyzed the macrophage-induced gene expression of three diverse genotypes of Mycobacterium avium subsp. paratuberculosis (MAP). Using selective capture of transcribed sequences (SCOTS) on three genotypically diverse MAP isolates from cattle, human, and sheep exposed to primary bovine monocyte derived macrophages for 48 It and 120 h we created and sequenced six cDNA libraries. Sequence annotations revealed that the cattle isolate up-regulated 27 and 241 genes; the human isolate up-regulated 22 and 53 genes, and the sheep isolate up-regulated 35 and 358 genes, at the two time points respectively. Thirteen to thirty-three percent of the genes identified did not have any annotated function. Despite variations in the genes identified, the patterns of expression fell into overlapping cellular functions as inferred by pathway analysis. For example, 10-12% of the genes expressed by all three strains at each time point were associated with cell-wall biosynthesis. All three strains of MAP studied up-regulated genes in pathways that combat oxidative stress, metabolic and nutritional starvation, and cell survival. Taken together, this comparative transcriptional analysis suggests that diverse MAP genotypes respond with similar modus operandi for survival in the host. (C) 2008 Elsevier Masson SAS. All rights reserved.

    Journal Title

    Microbes and Infection

    Volume

    10

    Issue/Number

    12-13

    Publication Date

    1-1-2008

    Document Type

    Article

    Language

    English

    First Page

    1274

    Last Page

    1282

    WOS Identifier

    WOS:000261368400007

    ISSN

    1286-4579

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