Dynamic Light Scattering as a Powerful Tool for Gold Nanoparticle Bioconjugation and Biomolecular Binding Studies

    Authors

    H. Jans; X. Liu; L. Austin; G. Maes;Q. Huo

    Comments

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    Abbreviated Journal Title

    Anal. Chem.

    Keywords

    SURFACE-PLASMON RESONANCE; PROTEIN-PROTEIN INTERACTIONS; HYPER-RAYLEIGH-SCATTERING; DNA HYBRIDIZATION; IMMUNOGLOBULIN-G; TECHNOLOGY; PROBES; IMMUNOASSAY; ANTIBODIES; PARTICLES; Chemistry, Analytical

    Abstract

    Dynamic light scattering (DLS) is an analytical tool used routinely for measuring the hydrodynamic size of nanoparticles and colloids in a liquid environment Gold nanoparticles (GNPs) are extraordinary light scatterers at or near their surface plasmon resonance wavelength. In this study, we demonstrate that DLS can be used as a very convenient and powerful tool for gold nanoparticle bioconjugation and biomolecular binding studies. The conjugation process between protein A and gold nanoparticles under different experimental conditions and the quality as well as the stability of the prepared conjugates were monitored and characterized systematically by DLS. Furthermore, the specific interactions between protein A-conjugated gold nanoparticles and a target protein, human IgG, can be detected and monitored in situ by measuring the average particle size change of the assay solution. For the first time, we demonstrate that DLS is able to directly and quantitatively measure the binding stoichiometry between a protein-conjugated GNP probe and a target analyte protein in solution.

    Journal Title

    Analytical Chemistry

    Volume

    81

    Issue/Number

    22

    Publication Date

    1-1-2009

    Document Type

    Article

    Language

    English

    First Page

    9425

    Last Page

    9432

    WOS Identifier

    WOS:000271662400029

    ISSN

    0003-2700

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