Prostasin inhibits cell invasion in human choriocarcinomal JEG-3 cells

    Authors

    X. J. Ma; Y. Y. Fu; Y. X. Li; L. M. Chen; K. Chai;Y. L. Wang

    Comments

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    Abbreviated Journal Title

    Histochem. Cell Biol.

    Keywords

    Prostasin; Cell invasion; Choriocarcinomal JEG-3 cell; MMPs/TIMPs; Integrins; TROPHOBLAST INVASION; SERINE-PROTEASE; MATRIX METALLOPROTEINASE-26; BLOOD-PRESSURE; RHESUS-MONKEY; 1ST TRIMESTER; GAMMA-SUBUNIT; EXPRESSION; IMPLANTATION; ACTIVATION; Cell Biology; Microscopy

    Abstract

    Controlled invasion of the uterine wall by the trophoblast cells is pivotal for the successful pregnancy, and various kinds of protease are involved in this process. Serine protease prostasin has been shown to participate in the proteolytic activation of epithelial sodium channel as well as cleavage of epidermal growth factor receptor extracellular domain in human epithelial cells. Its physiological significance in human placentation has been suggested but not validated. In the present study, we found that prostasin was expressed at a relatively high level in human placenta trophoblasts in early pregnant weeks. In the in vitro cultured human choriocarcinomal JEG-3 cells, treatment with functional antibody against prostasin led to promotion in cell invasion capability, as well as increase in the production of MMP-2, MMP-26, TIMP-1, and TIMP-4. Our data indicated that this serine protease may function as an invasion suppressor in human trophoblast, participating in the invasion-restrictive regulation of trophoblasts to avoid their over-penetration into the uterine wall.

    Journal Title

    Histochemistry and Cell Biology

    Volume

    132

    Issue/Number

    6

    Publication Date

    1-1-2009

    Document Type

    Article

    Language

    English

    First Page

    639

    Last Page

    646

    WOS Identifier

    WOS:000271764400007

    ISSN

    0948-6143

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