Title

Prostasin inhibits cell invasion in human choriocarcinomal JEG-3 cells

Authors

Authors

X. J. Ma; Y. Y. Fu; Y. X. Li; L. M. Chen; K. Chai;Y. L. Wang

Comments

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Abbreviated Journal Title

Histochem. Cell Biol.

Keywords

Prostasin; Cell invasion; Choriocarcinomal JEG-3 cell; MMPs/TIMPs; Integrins; TROPHOBLAST INVASION; SERINE-PROTEASE; MATRIX METALLOPROTEINASE-26; BLOOD-PRESSURE; RHESUS-MONKEY; 1ST TRIMESTER; GAMMA-SUBUNIT; EXPRESSION; IMPLANTATION; ACTIVATION; Cell Biology; Microscopy

Abstract

Controlled invasion of the uterine wall by the trophoblast cells is pivotal for the successful pregnancy, and various kinds of protease are involved in this process. Serine protease prostasin has been shown to participate in the proteolytic activation of epithelial sodium channel as well as cleavage of epidermal growth factor receptor extracellular domain in human epithelial cells. Its physiological significance in human placentation has been suggested but not validated. In the present study, we found that prostasin was expressed at a relatively high level in human placenta trophoblasts in early pregnant weeks. In the in vitro cultured human choriocarcinomal JEG-3 cells, treatment with functional antibody against prostasin led to promotion in cell invasion capability, as well as increase in the production of MMP-2, MMP-26, TIMP-1, and TIMP-4. Our data indicated that this serine protease may function as an invasion suppressor in human trophoblast, participating in the invasion-restrictive regulation of trophoblasts to avoid their over-penetration into the uterine wall.

Journal Title

Histochemistry and Cell Biology

Volume

132

Issue/Number

6

Publication Date

1-1-2009

Document Type

Article

Language

English

First Page

639

Last Page

646

WOS Identifier

WOS:000271764400007

ISSN

0948-6143

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