Title
Cationic polypeptides contribute to the anti-HIV-1 activity of human seminal plasma
Abbreviated Journal Title
Faseb J.
Keywords
antigens; epitopes; AIDS; reproductive immunology; viral; antimicrobial; IMMUNODEFICIENCY-VIRUS TYPE-1; POLYACRYLAMIDE-GEL ELECTROPHORESIS; PROSTATE-SPECIFIC ANTIGEN; SPERM MOTILITY INHIBITOR; ANTIMICROBIAL; PEPTIDES; SEMENOGELIN-I; ANTIBACTERIAL ACTIVITY; ANTIVIRAL ACTIVITY; PROTEIN; LACTOFERRIN; Biochemistry & Molecular Biology; Biology; Cell Biology
Abstract
Mucosal surfaces of the reproductive tract as well as their secretions have important roles in preventing sexual transmission of HIV-1. In the current study, the majority of the intrinsic anti-HIV-1 activity of human seminal plasma (SP) was determined to reside in the cationic polypeptide fraction. Antiviral assays utilizing luciferase reporter cells and lymphocytic cells revealed the ability of whole SP to prevent HIV-1 infection, even when SP was diluted 3200-fold. Subsequent fractionation by continuous flow acid-urea (AU)-PAGE and antiviral testing revealed that cationic polypeptides within SP were responsible for the majority of anti-HIV-1 activity. A proteomic approach was utilized to resolve and identify 52 individual cationic polypeptides that contribute to the aggregate anti-HIV-1 activity of SP. One peptide fragment of semenogelin I, termed SG-1, was purified from SP by a multistep chromatographic approach, protein sequenced, and determined to exhibit anti-HIV-1 activity against HIV-1. Anti-HIV-1 activity was transient, as whole SP incubated for prolonged time intervals exhibited a proportional decrease in anti-HIV-1 activity that was directly attributed to the degradation of semenogelin I peptides. Collectively, these results indicate that the cationic polypeptide fraction of SP is active against HIV-1, and that semenogelin-derived peptides contribute to the intrinsic anti-HIV-1 activity of SP.-Martellini, J. A., Cole, A. C., Venkataraman, N., Quinn, G. A., Svoboda, P., Gangrade, B. K., Pohl, J., Sorensen, O. E., Cole, A. M. Cationic polypeptides contribute to the anti-HIV-1 activity of human seminal plasma. FASEB J. 23, 3609-3618 (2009). www.fasebj.org
Journal Title
Faseb Journal
Volume
23
Issue/Number
10
Publication Date
1-1-2009
Document Type
Article
Language
English
First Page
3609
Last Page
3618
WOS Identifier
ISSN
0892-6638
Recommended Citation
"Cationic polypeptides contribute to the anti-HIV-1 activity of human seminal plasma" (2009). Faculty Bibliography 2000s. 1866.
https://stars.library.ucf.edu/facultybib2000/1866
Comments
Authors: contact us about adding a copy of your work at STARS@ucf.edu