Cationic polypeptides contribute to the anti-HIV-1 activity of human seminal plasma

    Authors

    J. A. Martellini; A. L. Cole; N. Venkataraman; G. A. Quinn; P. Svoboda; B. K. Gangrade; J. Pohl; O. E. Sorensen;A. M. Cole

    Comments

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    Abbreviated Journal Title

    Faseb J.

    Keywords

    antigens; epitopes; AIDS; reproductive immunology; viral; antimicrobial; IMMUNODEFICIENCY-VIRUS TYPE-1; POLYACRYLAMIDE-GEL ELECTROPHORESIS; PROSTATE-SPECIFIC ANTIGEN; SPERM MOTILITY INHIBITOR; ANTIMICROBIAL; PEPTIDES; SEMENOGELIN-I; ANTIBACTERIAL ACTIVITY; ANTIVIRAL ACTIVITY; PROTEIN; LACTOFERRIN; Biochemistry & Molecular Biology; Biology; Cell Biology

    Abstract

    Mucosal surfaces of the reproductive tract as well as their secretions have important roles in preventing sexual transmission of HIV-1. In the current study, the majority of the intrinsic anti-HIV-1 activity of human seminal plasma (SP) was determined to reside in the cationic polypeptide fraction. Antiviral assays utilizing luciferase reporter cells and lymphocytic cells revealed the ability of whole SP to prevent HIV-1 infection, even when SP was diluted 3200-fold. Subsequent fractionation by continuous flow acid-urea (AU)-PAGE and antiviral testing revealed that cationic polypeptides within SP were responsible for the majority of anti-HIV-1 activity. A proteomic approach was utilized to resolve and identify 52 individual cationic polypeptides that contribute to the aggregate anti-HIV-1 activity of SP. One peptide fragment of semenogelin I, termed SG-1, was purified from SP by a multistep chromatographic approach, protein sequenced, and determined to exhibit anti-HIV-1 activity against HIV-1. Anti-HIV-1 activity was transient, as whole SP incubated for prolonged time intervals exhibited a proportional decrease in anti-HIV-1 activity that was directly attributed to the degradation of semenogelin I peptides. Collectively, these results indicate that the cationic polypeptide fraction of SP is active against HIV-1, and that semenogelin-derived peptides contribute to the intrinsic anti-HIV-1 activity of SP.-Martellini, J. A., Cole, A. C., Venkataraman, N., Quinn, G. A., Svoboda, P., Gangrade, B. K., Pohl, J., Sorensen, O. E., Cole, A. M. Cationic polypeptides contribute to the anti-HIV-1 activity of human seminal plasma. FASEB J. 23, 3609-3618 (2009). www.fasebj.org

    Journal Title

    Faseb Journal

    Volume

    23

    Issue/Number

    10

    Publication Date

    1-1-2009

    Document Type

    Article

    Language

    English

    First Page

    3609

    Last Page

    3618

    WOS Identifier

    WOS:000270354300037

    ISSN

    0892-6638

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