Expression of prostasin serine protease and protease nexin-1 (PN-1) in rhesus monkey ovary during menstrual cycle and early pregnancy

    Authors

    H. Zhang; H. Y. Lin; Q. Yang; H. X. Wang; K. X. Chai; L. M. Chen;C. Zhu

    Comments

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    Abbreviated Journal Title

    J. Histochem. Cytochem.

    Keywords

    prostasin; protease nexin-1; ovary; primate; PLASMINOGEN-ACTIVATOR; GRANULOSA-CELLS; MACACA-MULATTA; INHIBITORS; CANCER; RAT; LOCALIZATION; IMPLANTATION; PURIFICATION; KALLIKREINS; Cell Biology

    Abstract

    Serine proteases and their cognate serpin-class inhibitors are involved in the controlled proteolytic events during follicular development, ovulation, formation, and maintenance of the corpus luteum (CL). In this study, we investigated the expression pat-terns of prostasin serine protease and protease nexin-1 (PN-1), a serine protease inhibitor also called serpin-E2, in rhesus monkey ovaries during the menstrual cycle and early pregnancy, by using in situ hybridization and immunohistochemistry. Expression of prostasin was localized in oocyte, granulosa cells, and/or theca cells of early antral follicles and antral follicles, with high levels observed in preovulatory follicles. Prostasin was also localized at high levels of abundance in the CL during the menstrual cycle and early pregnancy. During the menstrual cycle, PN-1 was coordinately localized with prostasin in oocytes, granulosa cells, and theca cells of antral follicles and preovulatory follicles and in the CL. In addition, the PN-1 expression level in macaque CL during early pregnancy increased as pregnancy proceeded. We propose that prostasin may be involved in follicular development, ovulation, and CL formation, whereas PN-1 may be present to regulate the proteolysis in these processes.

    Journal Title

    Journal of Histochemistry & Cytochemistry

    Volume

    55

    Issue/Number

    12

    Publication Date

    1-1-2007

    Document Type

    Article

    Language

    English

    First Page

    1237

    Last Page

    1244

    WOS Identifier

    WOS:000250997400007

    ISSN

    0022-1554

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