Title

Using gold nanoparticles to improve the recovery and the limits of detection for the analysis of monohydroxy-polycyclic aromatic hydrocarbons in urine samples

Authors

Authors

H. Y. Wang; W. B. Wilson;A. D. Campiglia

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

J. Chromatogr. A

Keywords

Monohydroxy-polycyclic aromatic hydrocarbons; Gold nanoparticles; SPE-HPLC; Urine; Fluorescence; PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE MICROEXTRACTION; DRINKING-WATER SAMPLES; FLUORESCENCE DETECTION; MASS-SPECTROMETRY; METABOLITES; EXTRACTION; EXPOSURE; 1-HYDROXYPYRENE; QUANTIFICATION; Biochemical Research Methods; Chemistry, Analytical

Abstract

We present a novel approach to improve the analytical figures of merit of solid-phase extraction high-performance liquid chromatography (SPE-HPLC) for the analysis of monohydroxy-polycyclic aromatic hydrocarbons in urine samples. The novel alternative substitutes the evaporation step that is currently used in SPE-HPLC methodology with a pre-concentration procedure that extracts metabolites with gold nanoparticles. The analytical potential of the new approach is evaluated with the following six metabolites: 9-hydroxyphenanthrene, 2-hydroxyfluorene, 1-hydroxypyrene, 6-hydroxychrysene, 3-hydroxybenzo[a]pyrene and 4-hydroxybenzo[a]pyrene. We demonstrate that the substitution of the evaporation step with the gold nanoparticles procedure improves the overall recoveries, the relative standard deviations of the average recoveries and the limits of detection of SPE-HPLC analysis. The overall recoveries of the studied metabolites varied from 59.7 +/- 3.6% (2-hydroxyfluorene) to 92.3 +/- 2.5% (6-hydroxychrysene). The relative standard deviations of the average recoveries were lower than 6%. The limits of detection were at the parts-per-trillion levels and varied from similar to 2 pg mL(-1) (6-hydroxychrysene) to similar to 18 pg mL(-1) (2-hydroxyfluorene). Published by Elsevier B.V.

Journal Title

Journal of Chromatography A

Volume

1216

Issue/Number

31

Publication Date

1-1-2009

Document Type

Article

Language

English

First Page

5793

Last Page

5799

WOS Identifier

WOS:000268353200002

ISSN

0021-9673

Share

COinS