Using gold nanoparticles to improve the recovery and the limits of detection for the analysis of monohydroxy-polycyclic aromatic hydrocarbons in urine samples

    Authors

    H. Y. Wang; W. B. Wilson;A. D. Campiglia

    Comments

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    Abbreviated Journal Title

    J. Chromatogr. A

    Keywords

    Monohydroxy-polycyclic aromatic hydrocarbons; Gold nanoparticles; SPE-HPLC; Urine; Fluorescence; PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE MICROEXTRACTION; DRINKING-WATER SAMPLES; FLUORESCENCE DETECTION; MASS-SPECTROMETRY; METABOLITES; EXTRACTION; EXPOSURE; 1-HYDROXYPYRENE; QUANTIFICATION; Biochemical Research Methods; Chemistry, Analytical

    Abstract

    We present a novel approach to improve the analytical figures of merit of solid-phase extraction high-performance liquid chromatography (SPE-HPLC) for the analysis of monohydroxy-polycyclic aromatic hydrocarbons in urine samples. The novel alternative substitutes the evaporation step that is currently used in SPE-HPLC methodology with a pre-concentration procedure that extracts metabolites with gold nanoparticles. The analytical potential of the new approach is evaluated with the following six metabolites: 9-hydroxyphenanthrene, 2-hydroxyfluorene, 1-hydroxypyrene, 6-hydroxychrysene, 3-hydroxybenzo[a]pyrene and 4-hydroxybenzo[a]pyrene. We demonstrate that the substitution of the evaporation step with the gold nanoparticles procedure improves the overall recoveries, the relative standard deviations of the average recoveries and the limits of detection of SPE-HPLC analysis. The overall recoveries of the studied metabolites varied from 59.7 +/- 3.6% (2-hydroxyfluorene) to 92.3 +/- 2.5% (6-hydroxychrysene). The relative standard deviations of the average recoveries were lower than 6%. The limits of detection were at the parts-per-trillion levels and varied from similar to 2 pg mL(-1) (6-hydroxychrysene) to similar to 18 pg mL(-1) (2-hydroxyfluorene). Published by Elsevier B.V.

    Journal Title

    Journal of Chromatography A

    Volume

    1216

    Issue/Number

    31

    Publication Date

    1-1-2009

    Document Type

    Article

    Language

    English

    First Page

    5793

    Last Page

    5799

    WOS Identifier

    WOS:000268353200002

    ISSN

    0021-9673

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