Expression Profiling of Nuclear Receptors in Human and Mouse Embryonic Stem Cells

    Authors

    C. Q. Xie; Y. Jeong; M. G. Fu; A. L. Bookout; M. T. Garcia-Barrio; T. W. Sun; B. H. Kim; Y. Xie; S. Root; J. F. Zhang; R. H. Xu; Y. E. Chen;D. J. Mangelsdorf

    Abbreviated Journal Title

    Mol. Endocrinol.

    Keywords

    NANOG GENE-EXPRESSION; OCT4 EXPRESSION; SELF-RENEWAL; DIVERGENT PATHS; ERR-BETA; DIFFERENTIATION; PLURIPOTENCY; MAINTAIN; ALPHA; LINES; Endocrinology & Metabolism

    Abstract

    Nuclear receptors (NRs) regulate gene expression in essential biological processes including differentiation and development. Here we report the systematic profiling of NRs in human and mouse embryonic stem cell (ESC) lines and during their early differentiation into embryoid bodies. Expression of the 48 human and mouse NRs was assessed by quantitative real-time PCR. In general, expression of NRs between the two human cell lines was highly concordant, whereas in contrast, expression of NRs between human and mouse ESCs differed significantly. In particular, a number of NRs that have been implicated previously as crucial regulators of mouse ESC biology, including ERR beta, DAX-1, and LRH-1, exhibited diametric patterns of expression, suggesting they may have distinct species-specific functions. Taken together, these results highlight the complexity of the transcriptional hierarchy that exists between species and governs early development. These data should provide a unique resource for further exploration of the species-specific roles of NRs in ESC self-renewal and differentiation. (Molecular Endocrinology 23: 724-733, 2009)

    Journal Title

    Molecular Endocrinology

    Volume

    23

    Issue/Number

    5

    Publication Date

    1-1-2009

    Document Type

    Article

    Language

    English

    First Page

    724

    Last Page

    733

    WOS Identifier

    WOS:000265593100015

    ISSN

    0888-8809

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