Abbreviated Journal Title

J. Biol. Chem.

Keywords

Monocyte Chemoattractant Protein-1; Adipose-Tissue; Insulin-Resistance; Ppar-Gamma; Transcription Factor; C/Ebp-Alpha; Obesity; Angiogenesis; Pathway; Differentiation; Biochemistry & Molecular Biology

Abstract

Adipogenesis is a key differentiation process relevant to obesity and associated diseases such as type 2 diabetes. This process involves temporally regulated genes controlled by a set of transcription factors, CCAAT/enhancer-binding proteins (C/EBP) beta, C/EBP delta, and C/EBP alpha and peroxisome proliferator-activated receptor gamma (PPAR gamma). Currently, PPAR gamma is universally accepted as the master regulator that is necessary and sufficient to induce adipogenesis as no known factor can induce adipogenesis without PPAR gamma. We present evidence that a novel zinc finger protein, MCP-1-induced protein (MCPIP), can induce adipogenesis without PPAR gamma. Classical adipogenesis-inducing medium induces MCP-1 production and expression of MCPIP in 3T3-L1 cells before the induction of the C/EBP family of transcription factors and PPAR gamma. Knockdown of MCPIP prevents their expression and adipogenesis as measured by expression of adipocyte markers and lipid droplet accumulation. Treatment of 3T3-L1 cells with MCP-1 or forced expression of MCPIP induces expression of C/EBP beta, C/EBP delta, C/EBP alpha, and PPAR gamma and adipogenesis without any other inducer. Forced expression of MCPIP induces expression of the C/EBP family of transcription factors and adipogenesis in PPAR gamma(-/-) mouse embryonic fibroblasts. Thus, MCPIP is a newly identified protein that can induce adipogenesis without PPAR gamma.

Journal Title

Journal of Biological Chemistry

Volume

284

Issue/Number

40

Publication Date

1-1-2009

Document Type

Article

Language

English

First Page

27620

Last Page

27628

WOS Identifier

WOS:000270232300063

ISSN

0021-9258

Download

Share

COinS