Title
A one-step highly sensitive method for DNA detection using dynamic light scattering
Abbreviated Journal Title
J. Am. Chem. Soc.
Keywords
NANOPARTICLE PROBES; GOLD NANOPARTICLES; HYBRIDIZATION; NANOCRYSTALS; MICROARRAYS; Chemistry, Multidisciplinary
Abstract
A one-step homogenous DNA detection method with high sensitivity was developed using gold nanoparticles (AuNPs) coupled with dynamic light scattering (DLS) measurement. Citrate-protected AuNPs with a diameter of 30 nm were first functionalized with two seats of single-stranded DNA probes and then used as optical probes for DNA detection. In the presence of target DNA, the hybridization between target DNA and the two nanoparticle probes caused the formation of nanoparticle dimers, timers, and oligomers. As a result the nanoparticle aggregation increased the average diameter of the whole nanoparticle population, which can be monitored simply by DLS measurement. A quantative correlaiton can be established between the average diameter of the nanoparticles and the target DNA concentration. This DLS-based assay is extremely easy to conduct and requires no additional seperation and amplification steps. The detection limit is around 1 pM, which is 4 orders of magnitude better than that of light-absorption-based methods. Single base pair mismatched DNAs can be readily discriminated from perfectly matched target DNAs using this assay.
Journal Title
Journal of the American Chemical Society
Volume
130
Issue/Number
26
Publication Date
1-1-2008
Document Type
Article
DOI Link
Language
English
First Page
8138
Last Page
+
WOS Identifier
ISSN
0002-7863
Recommended Citation
"A one-step highly sensitive method for DNA detection using dynamic light scattering" (2008). Faculty Bibliography 2000s. 254.
https://stars.library.ucf.edu/facultybib2000/254
Comments
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