Title

A one-step highly sensitive method for DNA detection using dynamic light scattering

Authors

Authors

Q. Dai; X. Liu; J. Coutts; L. Austin;Q. Huo

Comments

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Abbreviated Journal Title

J. Am. Chem. Soc.

Keywords

NANOPARTICLE PROBES; GOLD NANOPARTICLES; HYBRIDIZATION; NANOCRYSTALS; MICROARRAYS; Chemistry, Multidisciplinary

Abstract

A one-step homogenous DNA detection method with high sensitivity was developed using gold nanoparticles (AuNPs) coupled with dynamic light scattering (DLS) measurement. Citrate-protected AuNPs with a diameter of 30 nm were first functionalized with two seats of single-stranded DNA probes and then used as optical probes for DNA detection. In the presence of target DNA, the hybridization between target DNA and the two nanoparticle probes caused the formation of nanoparticle dimers, timers, and oligomers. As a result the nanoparticle aggregation increased the average diameter of the whole nanoparticle population, which can be monitored simply by DLS measurement. A quantative correlaiton can be established between the average diameter of the nanoparticles and the target DNA concentration. This DLS-based assay is extremely easy to conduct and requires no additional seperation and amplification steps. The detection limit is around 1 pM, which is 4 orders of magnitude better than that of light-absorption-based methods. Single base pair mismatched DNAs can be readily discriminated from perfectly matched target DNAs using this assay.

Journal Title

Journal of the American Chemical Society

Volume

130

Issue/Number

26

Publication Date

1-1-2008

Document Type

Article

Language

English

First Page

8138

Last Page

+

WOS Identifier

WOS:000257152800015

ISSN

0002-7863

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