Authors

M. Davila; A. R. Frost; W. E. Grizzle;R. Chakrabarti

Abbreviated Journal Title

J. Biol. Chem.

Keywords

ACTIN CYTOSKELETAL DYNAMICS; IN-VITRO INVASION; COFILIN PHOSPHORYLATION; PROTEIN-KINASE; METALLOPROTEINASE MT1-MMP; MATRIX METALLOPROTEINASES; ZINC-FINGER; ACTIVATION; RHO; LIM-KINASE-1; Biochemistry & Molecular Biology

Abstract

Mammalian LIM kinase 1 (LIMK1) is involved in reorganization of actin cytoskeleton through inactivating phosphorylation of the ADF family protein cofilin, which depolymerizes actin filaments. Maintenance of the actin dynamics in an ordered fashion is essential for stabilization of cell shape or promotion of cell motility depending on the cell type. These are the two key phenomena that may become altered during acquisition of the metastatic phenotype by cancer cells. Here we show that LIMK1 is overexpressed in prostate tumors and in prostate cancer cell lines, that the concentration of phosphorylated cofilin is higher in metastatic prostate cancer cells, and that a partial reduction of LIMK1 altered cell proliferation by arresting cells at G(2)/ M, changed cell shape, and abolished the invasiveness of metastatic prostate cancer cells. We also show that the ectopic expression of LIMK1 promotes acquisition of invasive phenotype by the benign prostate epithelial cells. Our data provide evidence of a novel role of LIMK1 in regulating cell division and invasive property of prostate cancer cells and indicate that the effect is not mediated by phosphorylation of cofilin. Our study correlates with the recent observations showing a metastasis-associated chromosomal gain on 7q11.2 in prostate cancer, suggesting a possible gain in LIMK1 DNA (7q11.23).

Journal Title

Journal of Biological Chemistry

Volume

278

Issue/Number

38

Publication Date

1-1-2003

Document Type

Article

Language

English

First Page

36868

Last Page

36875

WOS Identifier

WOS:000185318300120

ISSN

0021-9258

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