Stable expression of a biodegradable protein-based polymer in tobacco chloroplasts

    Authors

    C. Guda; S. B. Lee;H. Daniell

    Abbreviated Journal Title

    Plant Cell Reports

    Keywords

    chloroplast transformation; biopolymer; protein-based polymer; molecular; farming; FOREIGN GENE-EXPRESSION; BIOLISTIC DELIVERY; ESCHERICHIA-COLI; HIGHER-PLANTS; HYPEREXPRESSION; TRANSFORMATION; CELLS; DNA; Plant Sciences

    Abstract

    Bioelastic protein-based polymers (PBP) have several medical (prevention of post-surgical adhesions) and non-medical (biodegradable plastic) applications. This study compares expression levels of PBP genes (synthetic) integrated into the nuclear genome or the large single-copy (LSC) or inverted repeat (IR) region of the chloroplast genome in transgenic tobacco plants. Polymer transcripts accumulated up to 100-fold higher in the IR plants than in those of nuclear transgenic plants. Integration of foreign genes into all of the chloroplast genomes (homoplasmy) and higher levels of polymer transcripts were observed only in the IR and not in LSC transgenic plants. Expression of the polymer protein was further confirmed by Western blot analysis.

    Journal Title

    Plant Cell Reports

    Volume

    19

    Issue/Number

    3

    Publication Date

    1-1-2000

    Document Type

    Article

    Language

    English

    First Page

    257

    Last Page

    262

    WOS Identifier

    WOS:000084933700008

    ISSN

    0721-7714

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