Title

Multigene engineering: dawn of an exciting new era in biotechnology

Authors

Authors

H. Daniell;A. Dhingra

Comments

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Abbreviated Journal Title

Curr. Opin. Biotechnol.

Keywords

BIOSYNTHETIC-PATHWAY; PHOTOSYSTEM-II; MESSENGER-RNA; FOREIGN GENES; PLANTS; EXPRESSION; CHLOROPLASTS; PROTEIN; POLYHYDROXYBUTYRATE; TRANSLATION; Biochemical Research Methods; Biotechnology & Applied Microbiology

Abstract

Development of a rice variety enriched in provitamin A, the accumulation of polyhydroxybutyrate polyester in Arabidopsis nuclear transgenic plants (with enzymes targeted to chloroplasts in both), and the expression of bacterial operons via the chloroplast genome are recent landmark achievements in multigene engineering. Hyper-expression of transgenes has resulted in the formation of insecticidal protein crystals or inclusion bodies of pharmaceutical proteins in transgenic chloroplasts, achieving the highest level of transgene expression ever reported in transgenic plants. These achievements illustrate the potential of multigene engineering to realize benefits of the post-genomic revolution.

Journal Title

Current Opinion in Biotechnology

Volume

13

Issue/Number

2

Publication Date

1-1-2002

Document Type

Review

Language

English

First Page

136

Last Page

141

WOS Identifier

WOS:000174643900010

ISSN

0958-1669

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