Multigene engineering: dawn of an exciting new era in biotechnology

    Authors

    H. Daniell;A. Dhingra

    Comments

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    Abbreviated Journal Title

    Curr. Opin. Biotechnol.

    Keywords

    BIOSYNTHETIC-PATHWAY; PHOTOSYSTEM-II; MESSENGER-RNA; FOREIGN GENES; PLANTS; EXPRESSION; CHLOROPLASTS; PROTEIN; POLYHYDROXYBUTYRATE; TRANSLATION; Biochemical Research Methods; Biotechnology & Applied Microbiology

    Abstract

    Development of a rice variety enriched in provitamin A, the accumulation of polyhydroxybutyrate polyester in Arabidopsis nuclear transgenic plants (with enzymes targeted to chloroplasts in both), and the expression of bacterial operons via the chloroplast genome are recent landmark achievements in multigene engineering. Hyper-expression of transgenes has resulted in the formation of insecticidal protein crystals or inclusion bodies of pharmaceutical proteins in transgenic chloroplasts, achieving the highest level of transgene expression ever reported in transgenic plants. These achievements illustrate the potential of multigene engineering to realize benefits of the post-genomic revolution.

    Journal Title

    Current Opinion in Biotechnology

    Volume

    13

    Issue/Number

    2

    Publication Date

    1-1-2002

    Document Type

    Review

    Language

    English

    First Page

    136

    Last Page

    141

    WOS Identifier

    WOS:000174643900010

    ISSN

    0958-1669

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