Somatic embryogenesis in the woody legume Calliandra tweedii

    Authors

    S. Kumar; V. Agrawal;S. C. Gupta

    Comments

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    Abbreviated Journal Title

    Plant Cell Tissue Organ Cult.

    Keywords

    in vitro regeneration; tree tissue culture; CERCIS-CANADENSIS; REGENERATION; EMBRYOS; INVITRO; TREE; Biotechnology & Applied Microbiology; Plant Sciences

    Abstract

    In vitro somatic embryogenesis was established from 1-1.2 cm long internodal and petiolar segments excised from 20-year-old woody legume, Calliandra tweedii Benth trees. Within 6-7 weeks, globular, heart, torpedo-shaped and dicot embryos differentiated directly. Among the cytokinins and auxins tested, 1 muM 2iP in Murashige and Skoog's (MS) medium induced well-organized normal embryos in 71% of the internodal segments. Petiolar explants responded best on 0.5 muM NAA wherein 30% of the cultures developed normal embryos. Somatic embryos developed roots and shoots when they were retained for 90 days in old desiccated MS medium. Plants ready for transfer to soil were regenerated through somatic embryogenesis in 4-5 months. These grew well after transfer to soil.

    Journal Title

    Plant Cell Tissue and Organ Culture

    Volume

    71

    Issue/Number

    1

    Publication Date

    1-1-2002

    Document Type

    Article

    Language

    English

    First Page

    77

    Last Page

    80

    WOS Identifier

    WOS:000177102800010

    ISSN

    0167-6857

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