Seroreactivities against Saccharomyces cerevisiae and Mycobacterium avium subsp paratuberculosis p35 and p36 antigens in Crohn's disease patients

    Authors

    I. Shafran; C. Piromalli; J. W. Decker; J. Sandoval; S. A. Naser;F. A. K. El-Zaatari

    Abbreviated Journal Title

    Dig. Dis. Sci.

    Keywords

    Crohn's disease; M. paratuberculosis; ASCA; serologic markers; INFLAMMATORY-BOWEL-DISEASE; ANTIBODIES; UTILITY; Gastroenterology & Hepatology

    Abstract

    Crohn's disease (CD) is an idiopathic chronic inflammatory bowel disease (IBD). Accurate diagnosis of the disease is of great clinical importance to assess its prognosis and success of therapy. Recent studies have validated and confirmed the potential utility of anti-Saccharomyces cerevisiae (baker's yeast; ASCA) IgG/IgA antibodies and anti-M. avium ss. paratuberculosis p35/p36 antibodies, separately, as serological markers to identify patients with CD. The efficacy of these markers was evaluated in the same patients with Crohn's disease. The anti-ASCA IgA/IgG and the anti-M. avium ss. paratuberculosis p35/p36 antibodies were positive in 60% (36/60) and 86.7% (52/60) of CD patients, respectively. When all the serologic markers were considered, the sensitivity in detecting CD was increased to 95.0% (57/60); 21 of 24 ASCA-negative patients were p35/p36-positive and five of eight of p35/p36-negative patients were ASCA-positive. This investigation further establishes the utility of p35 and p36 recombinant clones for the diagnosis of CD, and reveals the complimentary role of ASCA and p35 and p36 for effective detection of CD. Larger studies are needed to investigate the combined use of these serologic markers for the diagnosis of CD.

    Journal Title

    Digestive Diseases and Sciences

    Volume

    47

    Issue/Number

    9

    Publication Date

    1-1-2002

    Document Type

    Article

    Language

    English

    First Page

    2079

    Last Page

    2081

    WOS Identifier

    WOS:000177345400032

    ISSN

    0163-2116

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