Title
The theta-defensin, retrocyclin, inhibits HIV-1 entry
Abbreviated Journal Title
Aids Res. Hum. Retrovir.
Keywords
IMMUNODEFICIENCY-VIRUS TYPE-1; RHESUS-MACAQUE LEUKOCYTES; ANTIMICROBIAL; PEPTIDES; REPLICATION; CELLS; ANTIBIOTICS; INFECTION; FUSION; BLOCK; Immunology; Infectious Diseases; Virology
Abstract
Retrocyclin is a circular antimicrobial 18-residue peptide encoded in the human genome by a theta-defensin pseudogene. In the human genome, the gene for retrocyclin is inactivated by an in-frame stop codon in its signal sequence but its mature coding sequence is intact. The peptide corresponding to the processed human retrocyclin, generated by solid phase peptide synthesis, inhibited replication of R5 and X4 strains of HIV-1 in human cells. Luciferase reporter virus and Vpr-BLaM entry assays were used to demonstrate that retrocyclin specifically blocked R5 and X4 HIV-1 replication at entry. Surface plasmon resonance demonstrated that retrocyclin bound to soluble CD4 and gp120, but gp120 cell-binding assays revealed that retrocyclin did not fully inhibit the binding of soluble CD4 to gp120. A fluorescent retrocyclin congener localized in cell-surface patches either alone or colocalized with CD4, CXCR4, and CCR5. In the aggregate, these results suggest that retrocyclin blocks an entry step in HIV-1 replication. Retrocyclin represents a new class of small molecule HIV-1 entry inhibitors.
Journal Title
Aids Research and Human Retroviruses
Volume
19
Issue/Number
10
Publication Date
1-1-2003
Document Type
Article
Language
English
First Page
875
Last Page
881
WOS Identifier
ISSN
0889-2229
Recommended Citation
"The theta-defensin, retrocyclin, inhibits HIV-1 entry" (2003). Faculty Bibliography 2000s. 3939.
https://stars.library.ucf.edu/facultybib2000/3939
Comments
Authors: contact us about adding a copy of your work at STARS@ucf.edu