Title
Formation of a beta(1) integrin signaling complex in Schwann cells is independent of Rho
Abbreviated Journal Title
Glia
Keywords
myelination; differentiation; focal adhesion; FOCAL ADHESION KINASE; MYELIN FORMATION; BASAL LAMINA; PERIPHERAL-NERVE; LD4 MOTIF; PAXILLIN; PROTEIN; CYTOSKELETAL; EXPRESSION; BETA-1-INTEGRIN; Neurosciences
Abstract
Schwann cell adhesion to basal lamina is essential for peripheral nerve development. beta(1) integrin receptors for extracellular matrix cooperate with other receptors to transmit signals that coordinate cell cycle progression and initiation of differentiation, including myelin-specific gene expression. In Schwann cell/sensory neuron co-cultures, beta(1) integrins complex with focal adhesion kinase (FAK), fyn kinase, paxillin, and schwannomin in response to basal lamina adhesion. To study the assembly of this signaling complex in Schwann cells (SCs), we induced 0, integrin clustering on suspended cells using an immobilized antibody and recovered a complex containing beta(1) integrin, FAK, paxillin, and schwannomin. In adherent subconfluent cells, the proteins colocalized to filopodia, ruffling membranes and focal contacts. We assessed the role of rhoGTPase in the process of integrin complex assembly by introducing C3 transferase (C3T), a rho inhibitor, into the cells. Although C3T caused dose-dependent morphological abnormalities, FAK, paxillin, and schwannomin were able to coimmunoprecipitate with beta(1) integrin. Additionally, colocalization of FAK, paxillin, and schwannomin with beta(1) integrin in filopodia and small focal contacts remained unchanged. We conclude that SCs do not require active rho to recruit signaling and structural proteins to beta(1) integrins clustered at the plasma membrane. Rho is required to establish large focal adhesions and to spread and stabilize plasma membrane extensions. (C) 2003 Wiley-Liss, Inc.
Journal Title
Glia
Volume
41
Issue/Number
1
Publication Date
1-1-2003
Document Type
Article
DOI Link
Language
English
First Page
94
Last Page
104
WOS Identifier
ISSN
0894-1491
Recommended Citation
"Formation of a beta(1) integrin signaling complex in Schwann cells is independent of Rho" (2003). Faculty Bibliography 2000s. 4062.
https://stars.library.ucf.edu/facultybib2000/4062