Abbreviated Journal Title
J. Bacteriol.
Keywords
NITRIC-OXIDE; FATTY-ACIDS; NONREPLICATING PERSISTENCE; HYPOXIC RESPONSE; IN-VITRO; EXPRESSION; INFECTION; GENE; BIOSYNTHESIS; MICROORGANISMS; Microbiology
Abstract
Mycobacterium tuberculosis enters the host by inhalation of an infectious aerosol and replicates in the alveolar macrophages until the host's immune defense causes bacteriostasis, which leads the pathogen to go into nonreplicative drug-resistant dormancy. The dormant pathogen can survive for decades till the host's immune system is weakened and active tuberculosis develops. Even though fatty acids are thought to be the major energy source required for the persistence phase, the source of fatty acids used is not known. We postulate that the pathogen uses triacylglycerol (TG) as a storage form of fatty acids. Little is known about the biosynthesis of TG in M. tuberculosis. We show that 15 mycobacterial genes that we identified as putative triacylglycerol synthase (tgs) when expressed in Escherichia coli showed TGS activity, and we report some basic catalytic characteristics of the most active enzymes. We show that several tgs genes are induced when the pathogen goes into the nonreplicative drug-resistant state caused by slow withdrawal of O-2 and also by NO treatment, which is known to induce dormancy-associated genes. The gene (Rv3130c) that shows the highest TGS activity when expressed in E. coli shows the highest induction by hypoxia and NO treatment. Biochemical evidence shows that TG synthesis and accumulation occur under both conditions. We conclude that TG may be a form of energy storage for use during long-term dormancy. Therefore, TG synthesis may be an appropriate target for novel antilatency drugs that can prevent the organism from surviving dormancy and thus assist in the control of tuberculosis.
Journal Title
Journal of Bacteriology
Volume
186
Issue/Number
15
Publication Date
1-1-2004
Document Type
Article
Language
English
First Page
5017
Last Page
5030
WOS Identifier
ISSN
0021-9193
Recommended Citation
Daniel, Jaiyanth; Deb, Chirajyoti; Dubey, Vinod S.; Sirakova, Tatiana D.; Abomoelak, Bassam; Morbidoni, Hector R.; and Kolattukudy, Pappachan E., "Induction of a novel class of diacylglycerol acyltransferases and triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into a dormancy-like state in culture" (2004). Faculty Bibliography 2000s. 4290.
https://stars.library.ucf.edu/facultybib2000/4290
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