Title

Lysozyme levels in the nasal secretions of patients with perennial allergic rhinitis and recurrent sinusitis

Authors

Authors

V. C. Kalfa; S. L. Spector; T. Ganz;A. M. Cole

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

Ann. Allergy Asthma Immunol.

Keywords

MAJOR BASIC-PROTEIN; ANTIMICROBIAL PEPTIDE; TRACHEAL EPITHELIUM; HOST; DEFENSE; LAVAGE FLUID; EOSINOPHIL; LACTOFERRIN; CHILDREN; MUCOSA; ASTHMA; Allergy; Immunology

Abstract

Background: The association of perennial allergic rhinitis (PAR) with recurrent sinusitis (RS) is well recognized. Anatomic abnormalities at the osteomeatal complex or ciliary dysfunction may play a significant role in some patients. However, for most patients with allergy, the determinants of RS are unknown. Objective: To determine whether altered concentrations of antimicrobial peptides and proteins, such as lysozyme, lactoferrin, human beta-defensin-2 (HBD-2), and human neutrophil peptides 1 to 3 (HNP-1 to 3), contribute to the development of RS in patients with PAR. Methods: Nasal secretions were collected by vacuum aspiration from 15 individuals with PAR+RS, 16 with PAR alone, and 16 controls. Lysozyme and lactoferrin levels were determined in nasal secretions by using quantitative enzyme-linked immunosorbent assay, and HBD-2 and HNP-1 to 3 levels were determined in nasal secretions by using semiquantitative Western blot analysis. Eosinophil-derived neurotoxin (EDN) levels were measured by using enzyme-linked immunosorbent assay as a marker of nasal eosinophilia in all 3 groups. Results: Levels of EDN were elevated significantly in patients with PAR+RS compared with controls. Lysozyme levels were decreased significantly in patients with PAR+RS compared with PAR alone or controls. Mean lysozyme levels were significantly lower in patients with EDN levels greater than 1,000 ng/mL vs those with levels of 1,000 ng/mL or less in the PAR+RS group. There were no statistically significant differences in lactoferrin, HBD-2, and HNP-1 to 3 levels among the 3 groups. Conclusions: The presence of eosinophils and their products and reduced lysozyme concentrations may be critical factors that predispose the airways of patients with PAR to RS.

Journal Title

Annals of Allergy Asthma & Immunology

Volume

93

Issue/Number

3

Publication Date

1-1-2004

Document Type

Article

Language

English

First Page

288

Last Page

292

WOS Identifier

WOS:000224064800016

ISSN

1081-1206

Share

COinS