Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn's disease

    Authors

    S. A. Naser; G. Ghobrial; C. Romero;J. F. Valentine

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    Lancet

    Keywords

    POLYMERASE-CHAIN-REACTION; LYMPH-NODES; TISSUE; IDENTIFICATION; MILK; SUSCEPTIBILITY; ASSOCIATION; MUTATION; BACILLI; COWS; Medicine, General & Internal

    Abstract

    Background Crohn's disease, a form of inflammatory bowel disease, resembles some aspects of tuberculosis, leprosy, and paratuberculosis. The role of Mycobacterium avium subspecies paratuberculosis (MAP) in Crohn's disease is controversial. Methods We tested for MAP by PCR and culture in buffy coat preparations from 28 individuals with Crohn's disease, nine with ulcerative colitis, and 15 without inflammatory bowel disease. Findings MAP DNA in uncultured buffy coats was identified by PCR in 13 (46%) individuals with Crohn's disease, four (45%) with ulcerative colitis, and three (20%) without inflammatory bowel disease. Viable MAP was cultured from the blood of 14 (50%) patients with Crohn's disease, two (22%) with ulcerative colitis, and none of the individuals without inflammatory bowel disease. Current use of immunosuppressive medication did not correlate with a positive MAP culture. Sequencing of PCR products from MAP cultures confirmed the presence of the MAP-specific IS900 fragment. Among 11 MAP isolates assessed, we identified nine strains that were not identical. Interpretation We detected viable MAP in peripheral blood in a higher proportion of individuals with Crohn's disease than in controls. These data contribute to the evidence that MAP might be a cause of Crohn's disease.

    Journal Title

    Lancet

    Volume

    364

    Issue/Number

    9439

    Publication Date

    1-1-2004

    Document Type

    Article

    Language

    English

    First Page

    1039

    Last Page

    1044

    WOS Identifier

    WOS:000223959300022

    ISSN

    0140-6736

    Share

    COinS