Title
Prostasin attenuates inducible nitric oxide synthase expression in lipopolysaccharide-induced urinary bladder inflammation
Abbreviated Journal Title
Am. J. Physiol.-Renal Physiol.
Keywords
glycosylphosphatidylinositol-anchored serine protease; cytokines; cyclooxygenase-2; ESCHERICHIA-COLI LIPOPOLYSACCHARIDE; TIGHT JUNCTION DYSFUNCTION; INCREASED INOS ACTIVITY; EPITHELIAL SODIUM-CHANNEL; HUMAN SERINE; PROTEINASE; SMOOTH-MUSCLE-CELLS; ENDOTOXEMIC MICE; OUTLET OBSTRUCTION; MOLECULAR-CLONING; TRACT-INFECTIONS; Physiology; Urology & Nephrology
Abstract
Prostasin is a glycosylphosphatidylinositol- anchored serine protease, with epithelial sodium channel activation and tumor invasion suppression activities. We identified the bladder as an expression site of prostasin. In the mouse, prostasin mRNA expression was detected by reverse transcription and real-time polymerase chain reaction in the bladder, and the prostasin protein was localized by immunohistochemistry in the urothelial cells. In mice injected intraperitoneally with bacterial lipopolysaccharide (LPS), bladder prostasin mRNA expression was downregulated, whereas the expression of inducible nitric oxide synthase ( iNOS), cyclooxygenase-2 (COX-2), interferon-gamma (IFN gamma), TNF-alpha, IL-1 alpha, and IL-6 was upregulated. Viral promoter-driven expression of the human prostasin homolog in the bladder of transgenic mice attenuated the LPS induction of iNOS but did not abolish the induction. LPS induction of COX-2, TNF-alpha, IL-1 alpha, and IL-6 expression, however, was not reduced by prostasin transgene expression. Liposome-mediated delivery of prostasin-expressing plasmid into mouse bladder produced similar attenuation effects on LPS-induced iNOS expression, while not affecting COX-2 or cytokine induction. Mice receiving plasmid expressing a catalytic mutant prostasin did not manifest the iNOS induction attenuation phenotype. We propose a proteolytic mechanism for prostasin to intercept cytokine signaling during LPS-induced bladder inflammation.
Journal Title
American Journal of Physiology-Renal Physiology
Volume
291
Issue/Number
3
Publication Date
1-1-2006
Document Type
Article
Language
English
First Page
F567
Last Page
F577
WOS Identifier
ISSN
1931-857X
Recommended Citation
"Prostasin attenuates inducible nitric oxide synthase expression in lipopolysaccharide-induced urinary bladder inflammation" (2006). Faculty Bibliography 2000s. 4666.
https://stars.library.ucf.edu/facultybib2000/4666
Comments
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